US 11,970,545 B2
T cell-antigen coupler with Y182T mutation and methods of uses thereof
Christopher W. Helsen, Oakville (CA); Jonathan Bramson, Oakville (CA); Anna Dvorkin-Gheva, Burlington (CA); Galina F. Denisova, Hamilton (CA); Ksenia Bezverbnaya, Hamilton (CA); and Kenneth Anthony Mwawasi, Vancouver (CA)
Assigned to McMaster University, Hamilton (CA)
Filed by McMaster University, Hamilton (CA)
Filed on Mar. 22, 2023, as Appl. No. 18/188,318.
Application 18/188,318 is a continuation of application No. 16/753,577, granted, now 11,643,472, previously published as PCT/CA2018/051290, filed on Oct. 12, 2018.
Claims priority of provisional application 62/571,354, filed on Oct. 12, 2017.
Prior Publication US 2023/0265207 A1, Aug. 24, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C07K 16/30 (2006.01); A61K 9/00 (2006.01); A61K 38/00 (2006.01); A61K 39/385 (2006.01); A61P 35/00 (2006.01); C07K 14/525 (2006.01); C07K 14/705 (2006.01); C07K 14/725 (2006.01); C07K 14/73 (2006.01); C07K 16/28 (2006.01)
CPC C07K 16/3061 (2013.01) [A61K 9/0019 (2013.01); A61K 38/00 (2013.01); A61K 39/385 (2013.01); A61P 35/00 (2018.01); C07K 14/525 (2013.01); C07K 14/7051 (2013.01); C07K 14/70514 (2013.01); C07K 14/70517 (2013.01); C07K 16/2809 (2013.01)] 19 Claims
 
1. An engineered γδ T cell comprising an expression vector comprising a nucleic acid encoding a T Cell-Antigen Coupler (TAC) polypeptide, the TAC polypeptide comprising:
(a) an antigen-binding antibody fragment or DARPin that selectively binds a target antigen;
(b) a humanized UCHT1 (huUCHT1) polypeptide comprising the amino acid sequence of SEQ ID NO: 28; and
(c) a T cell co-receptor domain polypeptide comprising a CD4 cytosolic domain and a CD4 transmembrane domain, wherein the TAC polypeptide is expressed by the engineered γδ T cell.