US 11,970,524 B2
Recombinant ROBO2 proteins, compositions, methods and uses thereof
Stephen Berasi, Arlington, MA (US); Janet Elizabeth Buhlmann, Brookline, MA (US); Nathan Higginson-Scott, Boston, MA (US); Michael Shamashkin, Woburn, MA (US); Matthew Russo, Silver Spring, MD (US); Stefano V. Gulla, Boston, MA (US); Zong Sean Juo, Cambridge, MA (US); Sreekumar R. Kodangattil, Lexington, MA (US); Weining Lu, Boston, MA (US); Xueping Fan, Boston, MA (US); and David J. Salant, Boston, MA (US)
Assigned to PFIZER INC., New York, NY (US); and BOSTON MEDICAL CENTER CORPORATION, Boston, MA (US)
Filed by Pfizer Inc., New York, NY (US); and Boston Medical Center Corporation, Boston, MA (US)
Filed on Dec. 17, 2020, as Appl. No. 17/125,903.
Application 17/125,903 is a continuation of application No. 15/995,286, filed on Jun. 1, 2018, granted, now 10,906,955.
Claims priority of provisional application 62/663,082, filed on Apr. 26, 2018.
Claims priority of provisional application 62/514,242, filed on Jun. 2, 2017.
Prior Publication US 2021/0214414 A1, Jul. 15, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C07K 14/705 (2006.01); A61K 38/17 (2006.01); A61P 13/12 (2006.01); C07H 21/04 (2006.01); C07K 19/00 (2006.01); C12N 15/09 (2006.01); C12N 15/11 (2006.01); C12N 15/52 (2006.01); C12N 15/63 (2006.01); A61K 38/00 (2006.01)
CPC C07K 14/70503 (2013.01) [A61K 38/17 (2013.01); A61P 13/12 (2018.01); C07H 21/04 (2013.01); C07K 19/00 (2013.01); C12N 15/09 (2013.01); C12N 15/11 (2013.01); C12N 15/52 (2013.01); C12N 15/63 (2013.01); A61K 38/00 (2013.01); C07K 2319/30 (2013.01); C07K 2319/31 (2013.01); C07K 2319/70 (2013.01)] 25 Claims
OG exemplary drawing
 
1. A recombinant Roundabout Receptor 2 (ROBO2) protein comprising (i) a portion of ROBO2 extracellular domain, and (ii) an immunoglobulin domain, wherein said portion of ROBO2 extracellular domain comprises:
a) a ROBO2 first immunoglobulin-like domain (Ig1) of SEQ ID NO:9,
b) an inter domain linker between first and second immunoglobulin-like domains (Ig1-Ig2 inter-domain linker) of SEQ ID NO:10,
c) a second immunoglobulin-like domain (Ig2) of SEQ ID NO:11, and
d) an inter-domain linker between second and third immunoglobulin-like domains (Ig2-Ig3 inter-domain linker) of SEQ ID NO:12,
and wherein the recombinant ROBO2 protein is devoid of the three fibronectin type III (FNIII) repeats of the ROBO2 extracellular domain.