	US 11,969,731 B2
	Devices and methods for rapid PCR
Kirk Max Ririe, Salt Lake City, UT (US); David E. Jones, Layton, UT (US); Christopher Paul Pasko, Salt Lake City, UT (US); Anson Cole Chamberlain, American Fork, UT (US); Derek David, Salt Lake City, UT (US); Aaron Wernerehl, Salt Lake City, UT (US); and Jonathan Allen Bruns, Salt Lake City, UT (US)
Assigned to Biofire Defense, LLC, Salt Lake City, UT (US)
Filed by BIOFIRE DEFENSE, LLC, Salt Lake City, UT (US)
Filed on Nov. 24, 2020, as Appl. No. 17/103,354.
Application 17/103,354 is a division of application No. 16/078,637, granted, now 10,875,026, previously published as PCT/US2017/018748, filed on Feb. 21, 2017.
Claims priority of provisional application 62/330,701, filed on May 2, 2016.
Claims priority of provisional application 62/409,829, filed on Oct. 18, 2016.
Prior Publication US 2021/0078009 A1, Mar. 18, 2021
Int. Cl. B01L 7/00 (2006.01); B01L 3/00 (2006.01); C12Q 1/686 (2018.01)

CPC B01L 7/525 (2013.01) [B01L 3/50273 (2013.01); C12Q 1/686 (2013.01); B01L 2200/0647 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0864 (2013.01); B01L 2300/0867 (2013.01); B01L 2300/18 (2013.01); B01L 2300/1816 (2013.01); B01L 2300/1822 (2013.01); B01L 2300/1827 (2013.01); B01L 2300/1861 (2013.01); B01L 2400/0481 (2013.01)]

11 Claims

1. A flexible sample container comprising:

a reaction chamber having an array of reaction wells, wherein each of the wells of the array is fluidly connected to a selectively openable and selectively sealable fill channel,

wherein the flexible sample container is fabricated from a first film layer, a second film layer at least partially bonded to the first film layer, an adhesive layer bonded to the second film layer and one surface of a card layer in which the reaction wells of the array are formed, and a second adhesive layer bonded to a third, exterior film layer and an opposite surface of the card layer, and

wherein the fill channel comprises an open space between the first and second film layers.