US 11,968,983 B2
Tobacco leaf foliar spraying substance and method for reducing harmful ingredients in cheroot and tobacco shreds
Chunlei Yang, Wuhan (CN); Jinpeng Yang, Wuhan (CN); Jun Zhou, Wuhan (CN); Mei Yang, Wuhan (CN); Yong Yang, Wuhan (CN); Jun Yu, Wuhan (CN); Zongping Li, Wuhan (CN); Xiongfei Rao, Wuhan (CN); Guangjiong Qin, Wuhan (CN); Baoming Qiao, Wuhan (CN); Ruoshi Bai, Wuhan (CN); Yanjun Ma, Wuhan (CN); Xianbao Deng, Wuhan (CN); Wenzhang Qin, Wuhan (CN); Kaixiao Fan, Wuhan (CN); Candong Deng, Wuhan (CN); Yongle Wei, Wuhan (CN); and Youlun Fan, Wuhan (CN)
Assigned to HUBEI INSTITUTE OF TOBACCO SCIENCE, Wuhan (CN)
Filed by Hubei Institute of Tobacco Science, Wuhan (CN)
Filed on Jun. 17, 2023, as Appl. No. 18/336,958.
Claims priority of application No. 202211040095.0 (CN), filed on Aug. 29, 2022.
Prior Publication US 2023/0329246 A1, Oct. 19, 2023
Int. Cl. A01G 22/45 (2018.01); A01N 25/04 (2006.01); A01N 31/04 (2006.01); A01N 31/16 (2006.01); A01N 35/06 (2006.01); A01N 43/90 (2006.01); A01N 65/12 (2009.01); A01N 65/20 (2009.01); A01N 65/44 (2009.01); A01P 21/00 (2006.01)
CPC A01N 65/20 (2013.01) [A01G 22/45 (2018.02); A01N 25/04 (2013.01); A01N 31/04 (2013.01); A01N 31/16 (2013.01); A01N 35/06 (2013.01); A01N 43/90 (2013.01); A01N 65/12 (2013.01); A01N 65/44 (2013.01); A01P 21/00 (2021.08)] 3 Claims
 
1. A method for reducing carcinogenic ingredients in flue-cured tobacco leaves, comprising a step of spraying a tobacco leaf foliar spraying substance, 5 days before harvesting during the tobacco leaf maturation stage;
wherein the tobacco leaf foliar spraying substance is for reducing harmful ingredients in cheroot, wherein the tobacco leaf foliar spraying substance comprises a lotus leaf extract, and the harmful chemical ingredients comprise N-nitrosonornicotine, 4-(N-methyl-nitrosamine)-1-(3-pyridinyl)-1-butanone, N-nitrosoanabasine and N-nitrosoanatabine, the lotus leaf extract is prepared by the following method:
mashing freshly harvested lotus leaves with clean water in a mass ratio of 1:3 in tissue masher, injecting the mixed suspension after mashing into a circular ultrasonic extraction machine, wherein operation parameters for ultrasonic extraction are as follows: an extraction temperature is room temperature of 25±5° C., ultrasonic power is 1000W, a rotation speed is 1200 rpm, and extraction time is 30±5 min; and then after ultrasonic treatment, filtering, and diluting filtrate,
wherein the tobacco leaf foliar spraying substance comprises the following components in percentage by weight: 0.001-0.04% of 4,5-dihydroblumel A, 0.001-0.04% of 3-oxo-α-inol and 0.001403% of dehydrovomifoliol, the harmful chemical ingredients comprise N-nitrosonomicotine, 4-(N-methyl-nitrosamine)-1-(3-pyridinyl)-1-butanone, N-nitrosoanabasine and N-nitrosoanatabine.