	US 11,968,963 B2
	Male arthropod killing factors and methods of use thereof
Seth Bordenstein, Nashville, TN (US); and Jessamyn Perlmutter, Nashville, TN (US)
Assigned to VANDERBILT UNIVERSITY, Nashville, TN (US)
Appl. No. 16/982,708
Filed by VANDERBILT UNIVERSITY, Nashville, TN (US)
PCT Filed Apr. 5, 2019, PCT No. PCT/US2019/025936 § 371(c)(1), (2) Date Sep. 21, 2020, PCT Pub. No. WO2019/195645, PCT Pub. Date Oct. 10, 2019.
Claims priority of provisional application 62/652,982, filed on Apr. 5, 2018.
Prior Publication US 2021/0000092 A1, Jan. 7, 2021
Int. Cl. C07H 21/02 (2006.01); A01K 67/033 (2006.01); C07K 14/195 (2006.01); C12N 15/85 (2006.01)

CPC A01K 67/0339 (2013.01) [C07K 14/195 (2013.01); C12N 15/85 (2013.01); A01K 2217/052 (2013.01); A01K 2227/706 (2013.01)]

19 Claims

1. A genetically modified arthropod, said arthropod comprising:

a gene encoding a male arthropod killing factor; and

a promoter operably linked to the gene encoding the male arthropod killing factor;

wherein the expression of the gene encoding the male arthropod killing factor in arthropod embryos causes a reduction in viable surviving male offspring in comparison to arthropod embryos not expressing the gene encoding the male arthropod killing factor; and

wherein the male arthropod killing factor comprises the amino acid sequence SEQ ID NO:1, or an amino acid sequence at least 60% identical to SEQ ID NO:1.