US 12,287,348 B2
Autosampler structure for determining a vertical/horizontal distance between a needle tip and a vial top surface
Iori Otomo, Kyoto (JP)
Assigned to SHIMADZU CORPORATION, Kyoto (JP)
Appl. No. 17/048,350
Filed by Shimadzu Corporation, Kyoto (JP)
PCT Filed Jan. 9, 2019, PCT No. PCT/JP2019/000284
§ 371(c)(1), (2) Date Oct. 16, 2020,
PCT Pub. No. WO2019/207844, PCT Pub. Date Oct. 31, 2019.
Claims priority of application No. 2018-081956 (JP), filed on Apr. 23, 2018.
Prior Publication US 2021/0181222 A1, Jun. 17, 2021
Int. Cl. G01N 35/10 (2006.01); G01N 30/24 (2006.01); G01N 30/02 (2006.01)
CPC G01N 35/1011 (2013.01) [G01N 30/24 (2013.01); G01N 2030/027 (2013.01)] 5 Claims
OG exemplary drawing
 
1. An autosampler comprising:
a vial arrangement portion configured to arrange a plurality of vials thereon;
a needle assembly including a needle holding portion for holding a needle with a tip of the needle facing vertically downward, the needle being configured to insert the tip into a vial arranged on the vial arrangement portion from above to suck a sample in the vial from the tip, the needle assembly being configured to move the needle holding portion in a vertical direction and a horizontal plane direction to move the needle in the vertical direction and the horizontal plane direction;
an imaging portion configured to be movable in the horizontal plane direction and the vertical direction in a state in which a positional relationship with the needle is maintained, the imaging portion being configured to image the tip of the needle and a top surface of a vial directly below the tip from a position obliquely above the tip of the needle;
a correlation storing portion configured to store a correlation between a position and a size of the top surface of the vial within a visual field image of the imaging portion and a vertical distance between the imaging portion and the vial;
a distance measuring portion configured to measure a horizontal distance and a vertical distance between the tip of the needle and a target position above a target vial which is a vial that the tip of the needle is to be reached, in a state in which a top surface of the target vial is within the visual field image of the imaging portion together with the tip of the needle, based on a position and a size of the top surface of the target vial within the visual field image, a positional relationship between the tip of the needle and the target position above the target vial within the visual field image, and the correlation stored in the correlation storing portion; and
a needle moving portion configured to control a moving mechanism to make the tip of the needle reach the target position, based on the horizontal distance and the vertical distance measured by the distance measuring portion.
 
5. An autosampler comprising:
a vial arrangement portion;
a sample rack on the vial arrangement portion, the sample rack having vial holders arranged in a matrix;
a target vial disposed in a vial holder of the vial holders arranged in a matrix;
a needle assembly including a needle holder and a needle held by the needle holder with a needle with a tip of the needle facing downward, the needle assembly being configured to move the needle holder in a vertical direction and a horizontal plane direction;
an image sensor secured to the needle assembly to maintain a positional relationship with the needle, the image sensor positioned obliquely above the tip of the needle and oriented with the tip of the needle and a top surface of the target vial in a field of view of the image sensor; and
a controller configured to:
store a correlation between a size and position of the top surface of the target vial as viewed by the image sensor and a vertical distance between the image sensor and the top surface of the target vial;
determine the vertical distance between the image sensor and the top surface of the target vial based on the size of top surface of the target vial as viewed by image sensor;
determine the horizontal distance between the tip of the needle and the center of the top surface of the target vial based on a relative positional relationship between the tip of the needle and the center of the top surface of the target vial as viewed by the image sensor and the determined vertical distance;
cause the needle holder to move horizontally to position the tip of the needle over the center of the top surface of the target vial based on the determined horizontal distance; and
cause the needle holder to move vertically.