US 12,287,329 B2
Colors for chromogenic IHC and ISH staining with multi-dye quinone methide and tyramide conjugates
Julia Ashworth-Sharpe, Tucson, AZ (US); Brian D. Kelly, Tucson, AZ (US); Mark Lefever, Oro Valley, AZ (US); and Nathan W. Polaske, Oracle, AZ (US)
Assigned to Ventana Medical Systems, Inc., Tucson, AZ (US)
Filed by Ventana Medical Systems, Inc., Tucson, AZ (US)
Filed on Oct. 27, 2020, as Appl. No. 17/081,523.
Application 17/081,523 is a continuation of application No. 16/226,427, filed on Dec. 19, 2018, granted, now 11,143,648.
Application 16/226,427 is a continuation of application No. PCT/EP2017/065795, filed on Jun. 27, 2017.
Claims priority of provisional application 62/355,398, filed on Jun. 28, 2016.
Prior Publication US 2021/0055285 A1, Feb. 25, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/533 (2006.01); C09B 11/24 (2006.01); C09B 23/08 (2006.01); G01N 33/532 (2006.01); G01N 33/58 (2006.01)
CPC G01N 33/533 (2013.01) [C09B 11/24 (2013.01); C09B 23/083 (2013.01); G01N 33/532 (2013.01); G01N 33/582 (2013.01); G01N 33/583 (2013.01)] 11 Claims
 
1. A two-chromogen conjugate including a terminal reactive group, comprising: (a) a first chromogen, and (b) a second chromogen, wherein the first and second chromogens are coupled via a lysine, and wherein the conjugate further comprises a single terminal reactive functional group coupled to the carboxyl group of the lysine, wherein the first chromogen is coupled to a first amine group of the lysine, wherein the second chromogen is coupled to the second amine group of the lysine, wherein the first and second chromogens are independently selected from the group consisting of a rhodamine or a derivative or an analog thereof, Dabsyl or a derivative or an analog thereof, Dabcyl or a derivative or an analog thereof, Cy3 or a derivative or an analog thereof, Cy7 or a derivative or an analog thereof, Cy3.5 or a derivative or an analog thereof, Cy3B or a derivative or an analog thereof, Cy5 or a derivative or an analog thereof, Cy5.5 or a derivative or an analog thereof, fluorescein or a derivative or an analog thereof, or TAMRA or a derivative or an analog thereof.