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1. An optical method for detecting the presence of a target analyte in a sample, the method comprising: a) moving automatically a first portion of a whole blood sample from a support pack to a first well in a disk in a detection instrument and moving automatically a second portion of the whole blood sample from the support pack to a reaction well in the support pack, the disk comprising dried chemistries for processing the sample, plasma separation features configured to separate whole blood into plasma, and a hematocrit channel configured to execute the hematocrit assay, the support pack comprising the whole blood sample, pipette tips, buffers, a reagent for processing the sample, reagent preparation wells, and a monolayer assay device configured to operate as a hematology slide; b) separating by the detection instrument plasma from the first portion of the whole blood sample in the disk while simultaneously moving reagent in the support pack for processing the plasma generated in the disk and diluting the second portion of the whole blood sample in the support pack; c) moving automatically by the detection instrument the plasma generated from the first portion of the whole blood sample from the disk to the support pack; d) diluting by the detection instrument the plasma generated from the first portion of the whole blood sample in the support pack; e) moving automatically by the detection instrument the plasma generated from the first portion of the whole blood sample from the support pack to the disk; and f) optically detecting by the detection instrument the presence or absence of said first target analyte, the detection instrument comprising a laser and at least one of a spectrophotometer, or a camera.
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