US 12,287,276 B2
Detecting device for detecting biological particles and detecting method thereof
Tzu-Keng Chiu, New Taipei (TW); Yu-Xian Zhu, Hsinchu County (TW); Chi-Kun Ohyang, Taoyuan (TW); and Cheng-Fang Yang, New Taipei (TW)
Assigned to LIQBIO BIOMEDICAL COMPANY LIMITED, New Taipei (TW)
Filed by Chi-Kun Ohyang, Taoyuan (TW)
Filed on Mar. 7, 2022, as Appl. No. 17/688,031.
Claims priority of application No. 110110518 (TW), filed on Mar. 24, 2021.
Prior Publication US 2022/0307984 A1, Sep. 29, 2022
Int. Cl. G01N 15/1434 (2024.01); G01N 15/14 (2024.01); G01N 21/64 (2006.01)
CPC G01N 15/1434 (2013.01) [G01N 15/1425 (2013.01); G01N 2021/6439 (2013.01); G01N 21/6456 (2013.01)] 13 Claims
OG exemplary drawing
 
1. A detecting device for detecting a plurality of biological particles, comprising:
a detecting carrier for placing the biological particles, wherein the biological particles comprise at least one kind of target biological particles;
an optical system comprising:
an excitation light source for providing an excitation light to illuminate the biological particles, wherein the at least one kind of target biological particles in the biological particles is illuminated and excited by the excitation light to generate an emission light;
a filter and spectroscope group comprising a beam splitter, wherein the beam splitter is located on a light path of the emission light and is adapted to separate the incident emission light into a first detecting light and a second detecting light that account for different proportions of the emission light and respectively emit the first detecting light and the second detecting light, wherein a ratio of the first detecting light to the emission light ranges between 0.1 and 0.5, and a ratio of the second detecting light to the emission light ranges between 0.5 and 0.9;
at least one photomultiplier tube for receiving the first detecting light, wherein the at least one photomultiplier tube moves relative to the detecting carrier to scan a plurality of regional positions on the detecting carrier, and the detecting carrier is fixed; when the at least one photomultiplier tube receives the first detecting light in at least one of the regional positions on the detecting carrier, the at least one photomultiplier tube generates a regional positioning signal comprising a regional position of the at least one kind of target biological particles on the detecting carrier; and
at least one charge-coupled device for receiving the second detecting light, wherein when the at least one charge-coupled device receives the second detecting light, the at least one charge-coupled device generates an image signal comprising an image position of the at least one kind of target biological particles on the detecting carrier; and
a processor electrically connected to the optical system and adapted to receive the regional positioning signal and the image signal, wherein when the processor receives the regional positioning signal, the processor transmits the regional positioning signal to the at least one charge-coupled device, so that the at least one charge-coupled device moves relative to the detecting carrier, which is fixed, to the regional positions based on the regional positioning signal and generates the image signal, and the at least one charge-coupled device receives the second detecting light to detect the image position of the at least one kind of target biological particles in the regional positions to generate an image signal, and the processor obtains a precise location of the at least one kind of target biological particles in the regional position on the detecting carrier based on the image signal.