US 12,287,240 B2
Device and method for spectral imaging
Yuval Garini, Koranit (IL); Irena Bronshtein-Berger, Jerusalem (IL); and Iris Barshack, Tel Aviv-Jaffa (IL)
Assigned to Bar-Han University, Ramat-Gan (IL); and Tel HaShomes Medical Research Infrastructure and Services Ltd., Ramat-Gan (IL)
Filed by Bar-Ilan University, Ramat-Gan (IL); and Tel HaShomer Medical Research Infrastructure and Services Ltd., Ramat-Gan (IL)
Filed on Jan. 24, 2022, as Appl. No. 17/582,741.
Application 17/582,741 is a continuation of application No. PCT/IL2020/050827, filed on Jul. 24, 2020.
Claims priority of provisional application 62/877,858, filed on Jul. 24, 2019.
Prior Publication US 2022/0146311 A1, May 12, 2022
Int. Cl. G01J 3/28 (2006.01); G01J 3/46 (2006.01); G01J 3/06 (2006.01)
CPC G01J 3/2823 (2013.01) [G01J 3/462 (2013.01); G01J 2003/066 (2013.01); G01J 2003/2826 (2013.01)] 10 Claims
OG exemplary drawing
 
1. A method of analyzing a spectral image of a sample having cells stained by a stain, the method comprising:
identifying in the spectral image a plurality of nuclei, and extracting, for each nucleus, a spectrum characterizing an optical transmission and absorbance of the nucleus within a wavelength range corresponding to the stain, thereby providing a plurality of spectra, one spectrum for each nucleus of at least a portion of the nuclei;
calculating mean square error relative to a reference spectrum and/or vectorial properties of the spectra in each dimension of a multi-dimensional vector representing the spectra in a multi-dimensional space;
comparing shapes of the spectra thereamongst based on said mean square error and/or said vectorial properties;
identifying each nucleus of the portion of the nuclei, as belonging to one of at least a first population of nuclei and a second population of nuclei based on the comparing; and
marking the nucleus according to the identification.