	US 12,286,675 B2
	Epigenetic markers for the identification of blood sub-cells of type 1
Sven Olek, Berlin (DE); and Ivana Turbachova, Berlin (DE)
Assigned to Precision for Medicine GmbH, Berlin (DE)
Filed by Precision for Medicine GmbH, Berlin (DE)
Filed on Apr. 9, 2019, as Appl. No. 16/379,260.
Application 16/379,260 is a continuation of application No. 15/477,768, filed on Apr. 3, 2017, abandoned.
Application 15/477,768 is a continuation in part of application No. 13/139,808, abandoned, previously published as PCT/EP2009/008764, filed on Dec. 8, 2009.
Claims priority of application No. 08021838 (EP), filed on Dec. 16, 2008.
Prior Publication US 2019/0241957 A1, Aug. 8, 2019
Int. Cl. C12Q 1/6881 (2018.01)

CPC C12Q 1/6881 (2013.01) [C12Q 2600/154 (2013.01); C12Q 2600/158 (2013.01)]

6 Claims

1. A method for producing an amplicon from a bisulfite treated human CD3 genetic region, the method comprising:

a) bisulfite treating genomic DNA obtained from a human sample to produce bisulfite treated DNA, and

b) amplifying a CD3 genetic region from the bisulfite treated DNA with at least one primer pair to produce the amplicon,

wherein the amplicon comprises the sequence of SEQ ID NO: 43, SEQ ID NO: 55, or SEQ ID NO: 60.