| CPC C12Q 1/686 (2013.01) [B01L 7/52 (2013.01); G01N 21/6452 (2013.01); B01L 9/06 (2013.01); B01L 2200/141 (2013.01); B01L 2200/142 (2013.01); B01L 2300/042 (2013.01); B01L 2300/0654 (2013.01); B01L 2300/0829 (2013.01); B01L 2300/0851 (2013.01); B01L 2300/1822 (2013.01); G01N 2021/6482 (2013.01); G01N 2021/6484 (2013.01)] | 24 Claims |

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1. An apparatus for performing nucleic acid amplification reactions, the apparatus comprising:
a thermally-conductive receptacle holder, the receptacle holder comprising a plurality of receptacle wells, each of the receptacle wells being configured to receive a receptacle, and each of the receptacle wells having a through-hole extending from an inner surface of the receptacle well to an outer surface of the receptacle holder;
a cover movable between an open position and a closed position relative to the receptacle holder, the cover being configured to seat or secure receptacles disposed within the receptacle wells when the cover moves from the open position to the closed position; and
a plurality of optical fibers, each of the optical fibers having a first end and a second end, the first end of each optical fiber being in optical communication with an associated one of the receptacle wells, and the second end of each optical fiber being in optical communication with at least one of an excitation signal source and an emission signal detector, wherein the first end of each of the optical fibers extends through the through-hole of the associated receptacle well and into the associated receptacle well when a receptacle is not present in the associated receptacle well and the cover is in the closed position, and wherein the first end of each of the optical fibers contacts a receptacle disposed in the associated receptacle well when the cover is in the closed position.
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