US 12,286,661 B2
Methods for making high intensity sweeteners
Andrew P. Patron, San Marcos, CA (US); Chris Edano Noriega, San Diego, CA (US); Rama Rao Manam, San Diego, CA (US); Justin Colquitt, San Diego, CA (US); Nathan Faber, San Diego, CA (US); Helge Zieler, Del Mar, CA (US); Justin Stege, San Diego, CA (US); and Daniel K. Olson, San Diego, CA (US)
Assigned to Firmenich Incorporated, Plainsboro, NJ (US)
Appl. No. 17/291,945
Filed by Firmenich Incorporated, Plainsboro, NJ (US)
PCT Filed Nov. 1, 2019, PCT No. PCT/US2019/059498
§ 371(c)(1), (2) Date May 6, 2021,
PCT Pub. No. WO2020/096907, PCT Pub. Date May 14, 2020.
Claims priority of provisional application 62/757,141, filed on Nov. 7, 2018.
Prior Publication US 2024/0218402 A1, Jul. 4, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 9/14 (2006.01); C12N 9/10 (2006.01); C12N 9/26 (2006.01); C12N 9/42 (2006.01); C12N 15/52 (2006.01); C12P 7/22 (2006.01)
CPC C12P 7/22 (2013.01) [C12N 9/1051 (2013.01); C12N 9/1074 (2013.01); C12N 9/2411 (2013.01); C12N 9/2437 (2013.01); C12N 15/52 (2013.01); C12Y 204/01005 (2013.01); C12Y 204/01019 (2013.01); C12Y 302/01004 (2013.01)] 20 Claims
 
1. A method of producing Compound 1 having the structure of:

OG Complex Work Unit Chemistry
the method comprising:
contacting mogroside IIIE with a recombinant host cell that comprises a first enzyme capable of catalyzing a production of Compound 1 from mogroside IIIE, wherein the recombinant host cell comprises:
(1) a cytochrome P450 that comprises an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 1025; and/or
(2) a cytochrome P450 that is encoded by a nucleic acid sequence having at least 90% sequence identity to SEQ ID NO: 1024; and/or
(3) a glycosylating enzyme that comprises an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 1051; and/or
(4) a glycosylating enzyme that is encoded a nucleic acid sequence having at least 90% sequence identity to SEQ ID NO: 1050; and/or
(5) an epoxide hydrolase that comprises an amino acid sequence having at least 90% sequence identity to the sequence of SEQ ID NO: 1073; and/or
(6) an epoxide hydrolase that is encoded by a nucleic acid sequence having at least 90% sequence identify to the sequence of SEQ ID NO: 1072.