US 12,286,648 B2
Engineered proline hydroxylase biocatalysts for hydroxylation of chemical compounds
Jovana Nazor, Milpitas, CA (US); Robert Osborne, Raleigh, NC (US); Jack Liang, South San Francisco, CA (US); Jonathan Vroom, South San Francisco, CA (US); Xiyun Zhang, Fremont, CA (US); David Entwistle, San Carlos, CA (US); Rama Voladri, Pleasanton, CA (US); Ravi David Garcia, Los Gatos, CA (US); Jeffrey C. Moore, Westfield, NJ (US); Shane Grosser, Princeton, NJ (US); Birgit Kosjek, Westfield, NJ (US); and Matthew Truppo, Ocean Township, NJ (US)
Assigned to Codexis, Inc., Redwood City, CA (US)
Filed by Codexis, Inc., Redwood City, CA (US)
Filed on Mar. 8, 2023, as Appl. No. 18/180,711.
Application 16/196,205 is a division of application No. 15/491,692, filed on Apr. 19, 2017, granted, now 10,184,117, issued on Jan. 22, 2019.
Application 18/180,711 is a continuation of application No. 17/072,910, filed on Oct. 16, 2020, granted, now 11,634,695.
Application 17/072,910 is a continuation of application No. 16/196,205, filed on Nov. 20, 2018, granted, now 10,844,358, issued on Nov. 24, 2020.
Claims priority of provisional application 62/347,724, filed on Jun. 9, 2016.
Prior Publication US 2023/0272354 A1, Aug. 31, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 9/02 (2006.01); C12P 17/12 (2006.01)
CPC C12N 9/0071 (2013.01) [C12P 17/12 (2013.01); C12Y 114/11 (2013.01); C12Y 114/11002 (2013.01)] 16 Claims
 
1. An engineered polypeptide having proline hydroxylase activity comprises an amino acid sequence having at least 90% sequence identity to reference sequence SEQ ID NO:604 and one or more residue differences as compared to SEQ ID NO:604 at residue positions selected from: 13, 14, 24, 26, 27, 30, 57, 61, 62, 72, 76, 77, 81, 82, 86, 88, 97, 114, 127, 128, 142, 158, 161, 163, 173, 175, 176, 178, 180, 184, 185, 186, 187, 188, 189, 191, 192, 195, 198, 200, 207, 209, 210, 211, 213, 215, 217, 218, 222, 225, 230, 233, 236, 238, 240, 241, 256, 259, 263, 265, 271, and 273.