US 12,286,618 B2
Lactic acid bacteria
Armelle Cochu-Blachére, Dangé-Saint-Romain (FR); Christophe Fremaux, Dangé-Saint-Romain (FR); Thomas Desfougéres, Dangé-Saint-Romain (FR); and Anaïs Jedrzejowski, Dangé-Saint-Romain (FR)
Assigned to International N&H Denmark Aps, Kongens Lyngby (DK)
Appl. No. 17/415,964
Filed by International N&H Denmark APS, Kongens Lyngby (DK)
PCT Filed Dec. 21, 2018, PCT No. PCT/EP2018/086681
§ 371(c)(1), (2) Date Jun. 18, 2021,
PCT Pub. No. WO2019/197051, PCT Pub. Date Oct. 17, 2019.
Prior Publication US 2022/0017855 A1, Jan. 20, 2022
Int. Cl. C12N 1/20 (2006.01); A23C 9/123 (2006.01); C07K 14/315 (2006.01); C12N 9/12 (2006.01)
CPC C12N 1/20 (2013.01) [A23C 9/1238 (2013.01); C07K 14/315 (2013.01); C12N 9/1205 (2013.01); C12Y 207/01002 (2013.01); A23V 2400/249 (2023.08)] 15 Claims
 
1. A lactose-positive, galactose-negative, Streptococcus thermophilus strain comprising:
at least one mutated gene encoding a protein of the mannose-glucose-specific phosphotransferase system (mannose-glucose-specific PTS), the glucose import activity of which is decreased or abolished, and
at least one mutated gene selected from the group consisting of:
a) a mutation in a glucokinase (glcK) gene such that the glucokinase activity of said strain is reduced but not null,
wherein the protein sequence of said glucokinase is a glcK variant sequence having at least 90% identity with SEQ ID NO:25 or SEQ ID NO:46,
wherein the amino acid of the glucokinase corresponding to position 275 of SEQ ID NO: 25 is not a glutamic acid, and/or the amino acid of the glucokinase corresponding to position 144 of SEQ ID NO:46 is not a glycine;
b) a mutation in a catabolite control protein A (ccpA) gene, wherein the sequence of said mutated ccpA gene is selected from the group consisting of:
i) a sequence comprising SEQ ID NO:71, and
ii) a ccpA variant sequence having at least 90% identity with SEQ ID NO:71; and
c) a combination thereof.