US 12,286,615 B2
Cell transfection by precise mechanical membrane disruption
Xiaoyun Ding, Superior, CO (US); and Apresio Kefin Fajrial, Boulder, CO (US)
Assigned to THE REGENTS OF THE UNIVERSITY OF COLORADO, A BODY CORPORATE, Denver, CO (US)
Filed by THE REGENTS OF THE UNIVERSITY OF COLORADO, A BODY CORPORATE, Denver, CO (US)
Filed on Jun. 12, 2023, as Appl. No. 18/208,548.
Application 18/208,548 is a continuation of application No. 16/875,283, filed on May 15, 2020, granted, now 11,685,891.
Claims priority of provisional application 62/849,420, filed on May 17, 2019.
Prior Publication US 2023/0340393 A1, Oct. 26, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12M 1/34 (2006.01); C12M 1/26 (2006.01); C12M 3/06 (2006.01); C12N 15/89 (2006.01); C12M 1/42 (2006.01); C12N 5/16 (2006.01)
CPC C12M 41/46 (2013.01) [C12M 23/16 (2013.01); C12M 33/04 (2013.01); C12N 15/89 (2013.01); C12M 35/04 (2013.01); C12N 5/16 (2013.01)] 22 Claims
OG exemplary drawing
 
1. A method, comprising:
a) providing;
i) a fluid comprising a nucleic acid and a plurality of target cells;
ii) a fluidic poration device comprising a plurality of channels, each of said plurality of channels having a surface;
iii) at least one nanostructure positioned on said surface;
b) introducing said fluid into said each of said plurality of channels;
c) piercing said each of said plurality of target cells the same number of times and to the same depth with said nanostructure;
d) transfecting said each of said plurality of target cells with an equivalent amount of said nucleic acid.