	US 12,286,611 B2
	Tissue culture vessel for preparation of compressed hydrogel skin grafts and related methods and systems
Claude Nicolas Holenstein, Zurich (CH); Vincent Ronfard, Villarzel (CH); Anna-Lena Dittrich, Zumikon (CH); Siegfried Graf, Kriens (CH); Christian Beyer, Olten (CH); Krzysztof Krasnopolski, Alpnach Dorf (CH); Diane Ledroit, Morges (CH); Gilles Weder, Cortaillod (CH); Roman Arnet, Ennetburgen (CH); Noa Schmid, Kriens (CH); and Charles Coen, Villars-sur-Glane (CH)
Assigned to Cutiss AG, Schlieren (CH)
Appl. No. 18/571,777
Filed by Cutiss AG, Schlieren (CH)
PCT Filed Jun. 20, 2022, PCT No. PCT/IL2022/050656 § 371(c)(1), (2) Date Dec. 19, 2023, PCT Pub. No. WO2022/269601, PCT Pub. Date Dec. 29, 2022.
Claims priority of provisional application 63/240,360, filed on Sep. 2, 2021.
Claims priority of provisional application 63/212,662, filed on Jun. 20, 2021.
Prior Publication US 2024/0263116 A1, Aug. 8, 2024
Int. Cl. C12M 3/00 (2006.01); C12M 1/00 (2006.01); C12M 1/04 (2006.01); C12N 5/071 (2010.01); C12N 5/077 (2010.01)

CPC C12M 21/08 (2013.01) [C12M 23/24 (2013.01); C12M 23/26 (2013.01); C12M 23/38 (2013.01); C12M 23/46 (2013.01); C12M 23/50 (2013.01); C12M 29/18 (2013.01); C12N 5/0629 (2013.01); C12N 5/0656 (2013.01); C12N 5/0698 (2013.01); C12N 2527/00 (2013.01); C12N 2539/00 (2013.01)]

13 Claims

1. A tissue culture vessel (100) comprising:

(a) a graft support tray (200);

(b) a box having a lid (400) and a base (300), said box engaging and retaining said tray;

wherein said tray has two operational states:

a first operational state in which a floor of said tray is slightly raised with respect to a floor of said base; and

a second operational state in which said floor of said tray descends to contact the floor of said base;

wherein said graft support tray (200; 201) comprises:

(a) a rigid frame (210);

(b) a liquid permeable membrane floor (220) attached to a lower edge (212) of said frame; and