US 12,285,529 B2
Method for preparing probiotic-loaded microcapsule, product obtained from the same, and use of the same
Gangqiang Ding, Xicheng District (CN); Junsheng Huo, Xicheng District (CN); Lianyan Wang, Haidian District (CN); Jian Huang, Xicheng District (CN); Guifeng Zhang, Haidian District (CN); Yanbin Tang, Xicheng District (CN); Ke Cao, Haidian District (CN); Yingbin Cui, Haidian District (CN); and Zheng Cao, Haidian District (CN)
Assigned to NATIONAL INSTITUTE FOR NUTRITION AND HEALTH CHINESE CENTER FOR DISEASE CONTROL AND PREVENTION, Beijing (CN)
Appl. No. 17/264,515
Filed by National Institute for Nutrition and Health Chinese Center for Disease Control and Prevention, Xicheng District (CN); and Institute of Process Engineering, Chinese Academy of Sciences, Haidian District (CN)
PCT Filed Aug. 18, 2020, PCT No. PCT/CN2020/109716
§ 371(c)(1), (2) Date Jan. 29, 2021,
PCT Pub. No. WO2021/088460, PCT Pub. Date May 14, 2021.
Claims priority of application No. 201911072088.7 (CN), filed on Nov. 5, 2019.
Prior Publication US 2022/0142933 A1, May 12, 2022
Int. Cl. A61K 9/50 (2006.01); A23L 29/256 (2016.01); A23L 33/135 (2016.01); A23P 10/30 (2016.01); A61K 35/745 (2015.01); A61K 35/747 (2015.01)
CPC A61K 9/5036 (2013.01) [A23L 29/256 (2016.08); A23L 33/135 (2016.08); A23P 10/30 (2016.08); A61K 35/745 (2013.01); A61K 35/747 (2013.01)] 15 Claims
OG exemplary drawing
 
1. A method for preparing a probiotic-loaded microcapsule, comprising the following steps:
(1) dissolving sodium alginate in a solvent to obtain a sodium alginate solution;
(2) evenly mixing a probiotic with the sodium alginate solution obtained in step (1) to obtain a probiotic suspension; and
(3) spraying the probiotic suspension obtained in step (2) into a salt solution, stirring and curing, to obtain the probiotic-loaded microcapsule,
wherein the probiotic suspension is sprayed into the salt solution at a gas velocity of 450-550 L/h and at a feed rate of 1-3 mL/min;
and wherein the probiotic in step (2) is selected from any one or a combination of Lactobacillus acidophilus, or Bifidobacterium, wherein the microcapsule has a particle size of 25-40 μm.