US 12,285,513 B2
Cell-free plant cell culture suspension supernatant with re-youth activity and/or wound healing activity over skin cells
Òscar Expósito Tarrés, Terrassa (ES); Albert Jané Font, Terrassa (ES); Sara Laplana Lasierra, Terrassa (ES); Maria Mas Duarte, Terrassa (ES); Tarik Ruiz Medina, Terrassa (ES); and Jessica Romero Rueda, Ripollet (ES)
Assigned to VYTRUS BIOTECH, S.L., Terrassa (ES)
Appl. No. 15/566,601
Filed by VYTRUS BIOTECH, S.L., Terrassa (ES)
PCT Filed Apr. 11, 2016, PCT No. PCT/EP2016/057885
§ 371(c)(1), (2) Date Oct. 13, 2017,
PCT Pub. No. WO2016/166047, PCT Pub. Date Oct. 20, 2016.
Claims priority of application No. 15163515 (EP), filed on Apr. 14, 2015.
Prior Publication US 2018/0133138 A1, May 17, 2018
Int. Cl. A61K 8/64 (2006.01); A61K 8/34 (2006.01); A61K 8/9789 (2017.01); A61K 8/9794 (2017.01); A61K 36/185 (2006.01); A61K 36/23 (2006.01); A61K 38/16 (2006.01); A61K 47/10 (2017.01); A61P 17/00 (2006.01); A61P 17/02 (2006.01); A61Q 19/00 (2006.01); A61Q 19/02 (2006.01); A61Q 19/08 (2006.01)
CPC A61K 8/645 (2013.01) [A61K 8/345 (2013.01); A61K 8/64 (2013.01); A61K 8/9789 (2017.08); A61K 8/9794 (2017.08); A61K 36/185 (2013.01); A61K 36/23 (2013.01); A61K 38/168 (2013.01); A61K 47/10 (2013.01); A61P 17/00 (2018.01); A61P 17/02 (2018.01); A61Q 19/00 (2013.01); A61Q 19/02 (2013.01); A61Q 19/08 (2013.01)] 6 Claims
 
1. A method for treating a skin wound in a human in need thereof comprising:
administering to the human in need thereof a therapeutically effective amount of a cell-free supernatant or a cell-free supernatant fraction, wherein said cell-free supernatant or a cell-free supernatant fraction thereof is obtained by a method comprising:
(i) growing dedifferentiated plant cells from a friable callus in a liquid nutrient culture medium for 5 days to 15 days, to obtain a conditioned media supporting the growth of the dedifferentiated plant cells; and
(ii) removing the entire plant cells from the conditioned media without applying a lysing step to the cells, to obtain the cell-free supernatant or the cell-free supernatant fraction comprising peptides from 4 amino acids to 300 amino acids in length, said peptides being selected from the group consisting of peptide plant growth factors, plant transcription factors, epigenetic factors, and mixtures thereof;
wherein the dedifferentiated plant cell culture suspension is from a plant selected from the group consisting of Daucus carota, Centella asiatica, Punica granatum, Gossypium herbaceum, Sarcocapnos crassifolia, Curcuma longa, Linum usitatissimum, Vitis vinifera, Lithops pseudotruncatella, Morinda citrifolia, Syvmphytum officinale, Cannabis sativa, Olea europaea, and Camellia sinensis.