US 12,285,473 B2
Cancer vaccines targeting MUC16 and uses thereof
Jian Yan, Wallingford, PA (US); Anna Slager, Lansdale, PA (US); Bradley Garman, Glenside, PA (US); and Neil Cooch, Oreland, PA (US)
Assigned to Inovio Pharmaceuticals, Inc., Plymouth Meeting, PA (US)
Filed by Inovio Pharmaceuticals, Inc., Plymouth Meeting, PA (US)
Filed on Dec. 22, 2021, as Appl. No. 17/559,824.
Application 17/559,824 is a division of application No. 16/219,460, filed on Dec. 13, 2018, granted, now 11,235,044.
Claims priority of provisional application 62/598,314, filed on Dec. 13, 2017.
Prior Publication US 2022/0111026 A1, Apr. 14, 2022
Int. Cl. C12N 15/00 (2006.01); A61K 39/00 (2006.01); C07K 14/47 (2006.01); C12N 15/86 (2006.01)
CPC A61K 39/00117 (2018.08) [C07K 14/4727 (2013.01); C07K 14/4748 (2013.01); A61K 2039/53 (2013.01); A61K 2039/54 (2013.01); A61K 2039/572 (2013.01)] 16 Claims
 
1. A nucleic acid molecule comprising one or more nucleic acid sequences selected from the group consisting of:
(a) a nucleic acid sequence that encodes amino acids 19-1490 of SEQ ID NO: 2;
(b) a nucleic acid sequence that encodes a protein that is at least 95% identical to amino acids 19-1490 of SEQ ID NO: 2, wherein the protein comprises at least one repeat micro-consensus (RMC) of RMC1-RMC4 of SEQ ID NO: 2;
(c) a nucleic acid sequence that encodes SEQ ID NO: 2;
(d) a nucleic acid sequence that encodes a protein that is at least 95% identical to SEQ ID NO: 2, wherein the protein comprises at least one RMC of RMC1-RMC4 of SEQ ID NO: 2;
(e) a nucleic acid sequence that encodes amino acids 19-642 of SEQ ID NO: 4;
(f) a nucleic acid sequence that encodes a protein that is at least 95% identical to amino acids 19-642 of SEQ ID NO: 4, wherein the protein comprises at least one RMC of RMC1-RMC4 of SEQ ID NO: 4;
(g) a nucleic acid sequence that encodes SEQ ID NO: 4;
(h) a nucleic acid sequence that encodes a protein that is at least 95% identical to SEQ ID NO: 4, wherein the protein comprises at least one RMC of RMC1-RMC4 of SEQ ID NO: 4;
(i) a nucleic acid sequence that encodes amino acids 19-710 of SEQ ID NO: 6;
(j) a nucleic acid sequence that encodes a protein that is at least 95% identical to amino acids 19-710 of SEQ ID NO: 6, wherein the protein comprises an alanine at amino acid position 628, an alanine at amino acid position 651, and an alanine at amino acid position 657 relative to SEQ ID NO: 6;
(k) a nucleic acid sequence that encodes SEQ ID NO: 6; and
(l) a nucleic acid sequence that encodes a protein that is at least 95% identical to SEQ ID NO: 6, wherein the protein comprises an alanine at amino acid position 628, an alanine at amino acid position 651, and an alanine at amino acid position 657 relative to SEQ ID NO: 6.