US 12,285,277 B2
Method and apparatus for medical imaging using differencing of multiple fluorophores
Kenneth Tichauer, Chicago, IL (US); Robert W. Holt, Lebanon, NH (US); Frederic Leblond, Montreal (CA); Pablo Valdes, Hanover, NH (US); Brian W. Pogue, Hanover, NH (US); Keith D. Paulsen, Hanover, NH (US); and David W. Roberts, Lyme, NH (US)
Assigned to The Trustees of Dartmouth College
Filed by The Trustees of Dartmouth College, Hanover, NH (US)
Filed on Mar. 25, 2024, as Appl. No. 18/614,944.
Application 18/614,944 is a continuation of application No. 18/159,495, filed on Jan. 25, 2023, granted, now 11,937,951.
Application 18/159,495 is a continuation of application No. 14/767,836, granted, now 11,564,639, issued on Jan. 31, 2023, previously published as PCT/US2014/016291, filed on Feb. 13, 2014.
Claims priority of provisional application 61/764,506, filed on Feb. 13, 2013.
Prior Publication US 2024/0260909 A1, Aug. 8, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. A61B 5/00 (2006.01); A61K 49/00 (2006.01)
CPC A61B 5/7278 (2013.01) [A61B 5/0071 (2013.01); A61K 49/0058 (2013.01); A61B 5/0035 (2013.01); A61B 5/0062 (2013.01); A61B 2576/00 (2013.01)] 19 Claims
OG exemplary drawing
 
1. A system for generating three dimensional images of bound fluorophore concentrations in tissue comprising:
a first apparatus configured for generating and illuminating tissue with light selectable between a nontargeted stimulus wavelength and a targeted stimulus wavelength;
a photodetector apparatus adaptable to capture nontargeted emissions data at nontargeted emission wavelengths from a nontargeted fluorophore in the tissue while the tissue is illuminated with light of the nontargeted stimulus wavelength, and to capture targeted emissions data at targeted emission wavelengths from a targeted fluorophore in the tissue while the tissue is illuminated with light of the targeted stimulus wavelength,
where at least the nontargeted emission wavelengths and the targeted emissions wavelengths are different;
a memory configured to store the nontargeted emissions data and the targeted emissions data;
a processor configured with machine readable code in the memory;
the machine readable code comprising instructions for reconstructing bound fluorophore concentrations of a voxel-based three dimensional model of distribution of bound targeted fluorophore concentration in tissue from difference data derived by subtracting the nontargeted emissions data from the targeted emissions data.