	US 12,285,013 B2
	Processing method for increasing seed production rate of seed fruits of Cucumis sativus L
Xiaodong Yang, Yangzhou (CN); Yizhuo Deng, Yangzhou (CN); Lili Zhang, Yangzhou (CN); Lei Qiu, Yangzhou (CN); Jieni Gu, Yangzhou (CN); and Xuehao Chen, Yangzhou (CN)
Assigned to Yangzhou University, Yangzhou (CN)
Filed by Yangzhou University, Yangzhou (CN)
Filed on Aug. 27, 2024, as Appl. No. 18/816,245.
Claims priority of application No. 202311174307.9 (CN), filed on Sep. 13, 2023.
Prior Publication US 2024/0415122 A1, Dec. 19, 2024
Int. Cl. A01N 43/66 (2006.01); A01H 3/04 (2006.01); A01N 25/30 (2006.01); A01P 21/00 (2006.01)

CPC A01N 43/66 (2013.01) [A01H 3/04 (2013.01); A01N 25/30 (2013.01); A01P 21/00 (2021.08)]

6 Claims

1. A method of preparing and applying an application of 5-azacytidine (C₈H₁₂N₄O₅) in increasing seed production efficiency of seed fruits of Cucumis sativus L., comprising a processing method for increasing seed production efficiency of seed fruits of Cucumis sativus L., wherein the method comprises the following steps:

(1) preparing a plant growth regulator according to a formula proportion;

preparing a 5-azacytidine solution with a concentration of 50 μM, adding an emulsifier Tween 80 at the same time, and mixing the 5-azacytidine solution and the emulsifier Tween 80 at a proportion of 10000:1 to obtain the plant growth regulator;

(2) selecting seed fruits of Cucumis sativus L. growing in a greenhouse and reaching a pollination stage, and dipping female flowers thereof with the plant growth regulator prepared in step (1) before pollination;

(3) picking male flowers for pollination after the female flowers are completely dry;

(4) dipping the pollinated female flowers again.