US 12,284,986 B2
Use of mouse model in evaluation of intelligence level
Cunxiang Ju, Nanjing (CN); Jing Zhao, Nanjing (CN); Jingjing Wang, Nanjing (CN); and Zhong Chen, Nanjing (CN)
Assigned to GEMPHARMATECH CO., LTD., Nanjing (CN)
Appl. No. 17/427,449
Filed by GEMPHARMATECH CO., LTD., Nanjing (CN)
PCT Filed Sep. 17, 2020, PCT No. PCT/CN2020/115844
§ 371(c)(1), (2) Date Jul. 30, 2021,
PCT Pub. No. WO2022/027798, PCT Pub. Date Feb. 10, 2022.
Claims priority of application No. 202010764676.3 (CN), filed on Aug. 3, 2020.
Prior Publication US 2023/0148569 A1, May 18, 2023
Int. Cl. A01K 29/00 (2006.01); A01K 67/02 (2006.01)
CPC A01K 29/005 (2013.01) [A01K 67/02 (2013.01); A01K 2227/105 (2013.01); A01K 2267/03 (2013.01)] 5 Claims
 
1. A method for evaluating safety of a material using a D000765 mouse model, the method comprising:
obtaining a resulting C57BL/6J mouse in which all or a portion of chromosome 1 is replaced by all or a portion of chromosome 1 from ZZ2 wild-type mouse;
crossbreeding the resulting C57BL/6J mouse with C57BL/6J mouse in which chromosome 1 has not been replaced to produce a mouse in which chromosome 1 is heterozygous while the remainder chromosomes are homozygous;
performing sibling selfing and screening by gene identification based on the mouse in which chromosome 1 is heterozygous while the remainder chromosomes are homozygous to obtain the D000765 mouse model, wherein the D000765 mouse model has increased learning ability, memory ability, and space exploration ability, when compared to a control wild-type C57BL/6J mouse;
administering the material to the D000765 mouse model;
determining whether the learning ability, memory ability, and space exploration ability of the D000765 mouse model is decreased after administration of the material, wherein a decrease in the learning ability, memory ability, and space exploration ability of the D000765 mouse model indicates that the material is not a safe material.