	US 12,284,977 B2
	Methods and compositions related to improved nitrogen utilization efficiency in tobacco
Jesse Frederick, Richmond, VA (US); Chengalrayan Kudithipudi, Midlothian, VA (US); and Dongmei Xu, Glen Allen, VA (US)
Assigned to Altria Client Services LLC, Richmond, VA (US)
Filed by Altria Client Services LLC, Richmond, VA (US)
Filed on Feb. 2, 2023, as Appl. No. 18/163,499.
Application 18/163,499 is a continuation of application No. 17/116,816, filed on Dec. 9, 2020, granted, now 11,602,118.
Application 17/116,816 is a continuation of application No. 16/119,366, filed on Aug. 31, 2018, granted, now 10,888,064, issued on Jan. 12, 2021.
Claims priority of provisional application 62/553,501, filed on Sep. 1, 2017.
Prior Publication US 2024/0099257 A1, Mar. 28, 2024
Int. Cl. A01H 6/82 (2018.01); A01H 5/10 (2018.01); A01H 5/12 (2018.01); C07K 14/415 (2006.01); C12N 9/10 (2006.01); C12N 9/88 (2006.01); C12N 9/90 (2006.01); C12N 15/82 (2006.01)

CPC A01H 6/823 (2018.05) [A01H 5/10 (2013.01); A01H 5/12 (2013.01); C07K 14/415 (2013.01); C12N 9/1085 (2013.01); C12N 9/88 (2013.01); C12N 9/90 (2013.01); C12N 15/8227 (2013.01); C12N 15/8237 (2013.01); C12N 15/8261 (2013.01); C12Y 205/01054 (2013.01); C12Y 402/01033 (2013.01); C12Y 501/03015 (2013.01)]

10 Claims

1. A method of producing a Nicotiana tabacum plant comprising an enhanced nitrogen utilization efficiency (NUE) trait, said method comprising:

a) selecting a Nicotiana tabacum plant comprising at least one favorable NUE allele comprising a T nucleotide at a position corresponding to position 36 of SEQ ID NO: 61 or a T nucleotide at a position corresponding to position 36 of SEQ ID NO: 64;

b) crossing said Nicotiana tabacum plant selected in step a) with a second Nicotiana tabacum plant; and

c) obtaining progeny seed from a cross of step b) wherein a plant grown from said progeny seed comprises said enhanced NUE trait and said at least one favorable NUE allele.