| CPC H04N 23/695 (2023.01) [G06T 7/215 (2017.01); G06T 7/337 (2017.01); H01J 37/20 (2013.01); G06T 2207/10061 (2013.01); H01J 2237/2594 (2013.01)] | 20 Claims |

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1. A method for measuring electron dose in a sample with a transmission electron microscope (TEM), the method comprising:
locating a fiducial mark on a TEM holder tip, wherein the TEM holder tip includes a through-hole located at a predetermined distance from the fiducial mark and a current collection area located at a predetermined distance from the fiducial mark;
calibrating the TEM for measuring beam area across a range of possible beam areas to generate a calibration table for beam area for the TEM;
calibrating the TEM for measuring beam current across a range of possible beam currents to generate a calibration table for beam current for the TEM; and
measuring electron dose on the sample during an experiment using the calibrated TEM having a defined configuration, wherein the measured electron dose is determined using the calibration table for beam area and the calibration table for beam current.
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