US 12,283,349 B2
Metagenomic analysis of microbial flora
Wataru Arai, Tokyo (JP); Yoshiyuki Sakuraba, Tokyo (JP); and Yoko Nagai, Tokyo (JP)
Assigned to VARINOS, INC., Tokyo (JP)
Appl. No. 18/549,570
Filed by Varinos, Inc., Tokyo (JP)
PCT Filed Dec. 15, 2021, PCT No. PCT/JP2021/046237
§ 371(c)(1), (2) Date Sep. 7, 2023,
PCT Pub. No. WO2022/190496, PCT Pub. Date Sep. 15, 2022.
Claims priority of application No. 2021-036093 (JP), filed on Mar. 8, 2021.
Prior Publication US 2024/0296909 A1, Sep. 5, 2024
Int. Cl. G06F 16/00 (2019.01); G06F 18/232 (2023.01); G16B 30/00 (2019.01); G16B 40/00 (2019.01)
CPC G16B 40/00 (2019.02) [G06F 18/232 (2023.01); G16B 30/00 (2019.02)] 11 Claims
OG exemplary drawing
 
1. A method of a metagenomic analysis of a bacterial flora, comprising:
performing clustering on base sequences obtained by DNA sequencing of a sample containing the bacterial flora, which will be hereinafter referred to as a sample sequence, wherein the clustering comprises
grouping base sequences having 100% similarity with each other, for the sample sequences thereby to generate a primary OTU (Operational Taxonomic Unit); and
performing the clustering on the sample sequences thereby to generate a secondary OTU composed of a sequence of a predetermined centroid, and sample sequences that have a similarity larger than or equal to a predetermined threshold value with the sequence of the centroid, on condition that the predetermined threshold value is less than 100%, and
setting a sequence of a primary OTU having a largest number of sequences among the primary OTUs included in each secondary OTU, as a representative sequence of the secondary OTU, collating the representative sequence with a database, and thereby estimating a lineage of the secondary OTU.