US 12,282,030 B2
Mass spectrometry assay for estrogenic compounds estradiol and estrone
Mildred Goldman, Laguna Niguel, CA (US); and Nigel J. Clarke, Vista, CA (US)
Assigned to Quest Diagnostics Investments LLC, Secaucus, NJ (US)
Filed by QUEST DIAGNOSTICS INVESTMENTS INCORPORATED, Wilmington, DE (US)
Filed on Nov. 12, 2020, as Appl. No. 17/096,301.
Application 17/096,301 is a continuation of application No. 15/251,224, filed on Aug. 30, 2016, granted, now 10,837,971, issued on Nov. 17, 2020.
Application 15/251,224 is a continuation of application No. 12/641,227, filed on Dec. 17, 2009, abandoned.
Claims priority of provisional application 61/161,160, filed on Mar. 18, 2009.
Claims priority of provisional application 61/140,575, filed on Dec. 23, 2008.
Prior Publication US 2021/0063418 A1, Mar. 4, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/68 (2006.01); G01N 30/72 (2006.01); G01N 33/74 (2006.01); H01J 49/00 (2006.01); H01J 49/26 (2006.01)
CPC G01N 33/743 (2013.01) [G01N 30/724 (2013.01); G01N 33/6848 (2013.01); G01N 33/6851 (2013.01); H01J 49/0027 (2013.01); G01N 2458/15 (2013.01); H01J 49/00 (2013.01)] 2 Claims
OG exemplary drawing
 
1. A method for determining the amount of 17β-estradiol and estrone in a human serum or plasma sample by mass spectrometry, the method comprising:
adding one or more deuterated internal standards of 17β-estradiol and/or estrone to a human serum or plasma sample containing 17β-estradiol and estrone to form a second sample;
subjecting the second sample to turbulent flow liquid chromatography to form a third sample;
purifying the third sample by high performance liquid chromatography to form a fourth sample;
ionizing the fourth sample with electrospray ionization to produce one or more ions of 17β-estradiol and estrone detectable by mass spectrometry;
determining the amounts of one or more ions of 17β-estradiol and estrone by tandem mass spectrometry, wherein the one or more ions of 17β-estradiol comprise an ion having a m/z ratio of 169.10±0.50; and
using the amounts of one or more ions and the amount of the ion having a m/z ratio of 169.10±0.50 from each of 17β-estradiol and estrone to determine the amounts of each of 17β-estradiol and estrone in the human serum or plasma sample.