US 12,282,024 B2
Evaluating method of ketosis in postpartum dairy cows
Takashi Mikami, Kanagawa (JP); Akira Imaizumi, Kanagawa (JP); Takayuki Tanaka, Kanagawa (JP); Yuki Miyazawa, Kanagawa (JP); Mina Nakamura, Kanagawa (JP); Kazuki Nakagawa, Kanagawa (JP); and Takeshi Fujieda, Kanagawa (JP)
Assigned to Ajinomoto Co., Inc., Tokyo (JP)
Filed by AJINOMOTO CO., INC., Tokyo (JP)
Filed on Feb. 18, 2021, as Appl. No. 17/178,805.
Application 17/178,805 is a continuation of application No. 16/233,032, filed on Dec. 26, 2018, granted, now 10,962,547.
Application 16/233,032 is a continuation of application No. PCT/JP2017/022927, filed on Jun. 21, 2017.
Claims priority of application No. 2016-130927 (JP), filed on Jun. 30, 2016.
Prior Publication US 2021/0172958 A1, Jun. 10, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/68 (2006.01); G01N 33/62 (2006.01); G01N 33/66 (2006.01); G01N 33/92 (2006.01)
CPC G01N 33/68 (2013.01) [G01N 33/62 (2013.01); G01N 33/66 (2013.01); G01N 33/92 (2013.01)] 3 Claims
 
1. An evaluating method comprising:
(A) evaluating a state of ketosis in a dairy cow after parturition by causing a control unit to execute evaluating a state of ketosis using T-Bil and at least one value of concentration values of Ala, Arg, Asn, Asp, BCAA, Cit, Cys, Glu, Gln, Gly, His, Ile, Leu, Lys, Met, Orn, Phe, Pro, Ser, Tau, Thr, Trp, Tyr, and Val and concentration values of ALB, ALT, AST, BHBA, BUN, Ca, gGTP, Glc, NEFA, TCHO, TG, and TP in blood of the dairy cow, wherein said values are obtained for said cow before the parturition, the evaluating (A) does not use a concentration value of 3MeHis, said evaluating (A) comprises
(i) evaluating by the control unit a value of a linear expression having the following explanatory variables: (a) a first explanatory value substituted with a concentration value of T-Bil in the cow; (b) a second explanatory value substituted with a concentration value of at least one Ala, Arg, Asn, Asp, BCAA, Cit, Cys, Glu, Gln, Gly, His, Ile, Leu, Lys, Met, 3, Orn, Phe, Pro, Ser, Tau, Thr, Trp, Tyr, and Val in the cow and (c) a third explanatory value substituted with a concentration value of at least one of ALB, ALT, AST, BHBA, BUN, Ca, gGTP, Glc, NEFA, TCHO, TG, and TP in the cow;
(ii) determining that the cow belongs a healthy group (BHBA<1200 μmol/l) or a ketosis group (BHBA≥1200 μmol/1) based on the evaluated value of the linear expression, and
(B) administering a prophylactic nutritional intervention before the parturition to the cow determined to belong to the ketosis group.