US 12,281,356 B2
Chemical compositions and methods of using same
Dwayne Dunaway, Seattle, WA (US); Elizabeth A. Manrao, Lake Forest Park, WA (US); Joseph M. Beechem, Piedmont, CA (US); Rustem Khafizov, Seattle, WA (US); Sanghamithra Korukonda, Seattle, WA (US); Yi Deng, Seattle, WA (US); Dae Kim, Bellevue, WA (US); Mark Gregory, Boise, ID (US); Margaret Hoang, Seattle, WA (US); Matthew Walsh, Seattle, WA (US); Gavin Meredith, Seattle, WA (US); Mark McElwain, Seattle, WA (US); Peter Skene, Issaquah, WA (US); and Cassandra Burke, Seattle, WA (US)
Assigned to Bruker Spatial Biology, Inc., Seattle, WA (US)
Filed by Bruker Spatial Biology, Inc., Seattle, WA (US)
Filed on Dec. 21, 2022, as Appl. No. 18/069,565.
Application 18/069,565 is a continuation of application No. 16/411,394, filed on May 14, 2019, granted, now 11,549,139.
Claims priority of provisional application 62/836,327, filed on Apr. 19, 2019.
Claims priority of provisional application 62/671,091, filed on May 14, 2018.
Prior Publication US 2023/0220461 A1, Jul. 13, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/68 (2018.01); C12Q 1/6839 (2018.01); C12Q 1/6869 (2018.01); C12Q 1/6876 (2018.01)
CPC C12Q 1/6869 (2013.01) [C12Q 1/6839 (2013.01); C12Q 1/6876 (2013.01)] 19 Claims
OG exemplary drawing
 
1. A probe comprising:
a target binding domain and a barcode domain;
wherein the target binding domain is at least 12 nucleotides in length;
wherein the barcode domain comprises a synthetic backbone, the barcode domain comprising at least two attachment positions, each attachment position comprising at least one attachment region comprising at least one nucleic acid sequence that hybridizes to a complementary nucleic acid molecule, and wherein the synthetic backbone comprises L-DNA,
wherein each of the at least two attachment positions has a different nucleic acid sequence,
wherein the at least two attachment positions correspond to the sequence of the target binding domain,
wherein said nucleic acid sequence of each position of the at least two attachment positions determines the identity of the target nucleic acid that is bound by said target binding domain, and
wherein each nucleotide of the at least one nucleic acid sequence of each attachment region is L-DNA.