	US 12,281,353 B1
	Terephthalate biosensors and uses thereof
Ramesh K. Jha, Los Alamos, NM (US); Taraka T. Dale, Los Alamos, NM (US); Ryan E. Bermel, Los Alamos, NM (US); and Ellen Lee Neidle, Athens, GA (US)
Assigned to TRIAD NATIONAL SECURITY, LLC, Los Alamos (MX); and UNIVERSITY OF GEORGIA RESEARCH FOUNDATION, INC., Athens, GA (US)
Filed by Triad National Security, LLC, Los Alamos, NM (US); and University of Georgia Research Foundation Inc., Athens, GA (US)
Filed on Feb. 10, 2021, as Appl. No. 17/173,065.
Claims priority of provisional application 62/972,365, filed on Feb. 10, 2020.
Int. Cl. C12Q 1/6825 (2018.01); C07K 14/47 (2006.01); C12Q 1/6853 (2018.01); G01N 33/58 (2006.01)

CPC C12Q 1/6825 (2013.01) [C07K 14/4702 (2013.01); C12Q 1/6853 (2013.01); G01N 33/582 (2013.01)]

13 Claims

1. A terephthalic acid (TPA) biosensor comprising a nucleic acid encoding a TPA responsive transcription factor from Comamonas testosteroni (TphR) protein, a TphR regulated promoter comprising the nucleic acid sequence of nucleotides 4-114 of SEQ ID NO: 12, and a nucleic acid encoding a reporter protein operably linked to the promoter.