US 12,281,349 B2
Recombinant vector comprising gene for synthesizing high molecular weight copolymer, transformant and method for producing polymers using transformant, recombinant strains having gene for synthesizing high molecular weight copolymers in genome and method for producing polymers using recombinant strain
Kozo Inoue, Tokyo (JP); K Sudesh Kumar C Kanapathi Pillai, Penang (MY); Manoj Kumar Lakshmanan, Penang (MY); and Hua Tiang Tan, Penang (MY)
Assigned to FUENCE CO., LTD., Tokyo (JP)
Appl. No. 17/606,022
Filed by FUENCE CO., LTD., Tokyo (JP)
PCT Filed Apr. 24, 2020, PCT No. PCT/JP2020/017812
§ 371(c)(1), (2) Date Oct. 23, 2021,
PCT Pub. No. WO2020/218566, PCT Pub. Date Oct. 29, 2020.
Claims priority of application No. 2019-086890 (JP), filed on Apr. 26, 2019.
Prior Publication US 2023/0313240 A1, Oct. 5, 2023
Int. Cl. C12P 7/625 (2022.01); C12N 9/10 (2006.01); C12N 9/88 (2006.01)
CPC C12P 7/625 (2013.01) [C12N 9/1029 (2013.01); C12N 9/88 (2013.01); C12Y 402/01017 (2013.01)] 18 Claims
 
1. A recombinant vector, which is a plasmid or phage, comprising:
an isolated polynucleotide encoding for a polypeptide with polymer synthase activity comprising an amino acid sequence set forth in SEQ ID NO: 1 or an amino acid sequence set forth in SEQ ID NO: 1,
wherein only one of amino acids thereof is replaced, deleted or added, and
an isolated polynucleotide encoding for a polypeptide with hydratase activity comprising an amino acid sequence set forth in SEQ ID NO: 3 or an amino acid sequence set forth in SEQ ID NO: 3, wherein only one of amino acids thereof is replaced, deleted or added.