US 12,281,347 B2
Method for producing 3-hydroxyisobutyric acid ester and methacrylic ester
Kenji Oki, Tokyo (JP); Fujio Yu, Tokyo (JP); Kozo Murao, Tokyo (JP); Yasuhisa Asano, Toyama (JP); and Fumihiro Motojima, Toyama (JP)
Assigned to Mitsubishi Chemical Corporation, Tokyo (JP); and Toyama Prefectural University, Toyama (JP)
Filed by Mitsubishi Chemical Corporation, Tokyo (JP); and Toyama Prefectural University, Toyama (JP)
Filed on Aug. 31, 2020, as Appl. No. 17/006,976.
Application 17/006,976 is a continuation of application No. PCT/JP2019/008098, filed on Mar. 1, 2019.
Claims priority of application No. 2018-037048 (JP), filed on Mar. 2, 2018.
Prior Publication US 2020/0392546 A1, Dec. 17, 2020
Int. Cl. C12P 7/62 (2022.01); C12N 1/21 (2006.01); C12N 9/10 (2006.01)
CPC C12P 7/62 (2013.01) [C12N 9/1029 (2013.01); C12Y 203/01084 (2013.01)] 9 Claims
 
1. A method for producing a 3-hydroxyisobutyric acid ester, comprising reacting an alcohol or phenol with 3-hydroxyisobutyryl-CoA in the presence of an alcohol acyltransferase to produce a 3-hydroxyisobutyric acid ester, wherein
the alcohol acyltransferase is any one of the following proteins (1) to (3):
(1) a protein comprising the amino acid sequence of SEQ ID NO:1;
(2) a protein comprising an amino acid sequence derived from the amino acid sequence of the SEQ ID NO:1 by deletion, insertion, substitution and/or addition of one or several amino acids, and having an alcohol acyltransferase activity catalyzing esterification of 3-hydroxyisobutyryl-CoA; and
(3) a protein comprising an amino acid sequence having a sequence identity of 80% or more with the amino acid sequence of SEQ ID NO:1 and having an alcohol acyltransferase activity that catalyzes esterification of 3-hydroxyisobutyryl-CoA,
wherein the presence of alcohol acyltransferase is provided by a genetically modified microorganism expressing the alcohol acyltransferase.