	US 12,281,343 B2
	Biotechnological production of diols
Dirk Fabritius, Ansbach (DE); Stefan Lambrecht, Hehlen (DE); Johannes Panten, Höxter (DE); and Marcus Eh, Holzminden (DE)
Assigned to SYMRISE AG, Holzminden (DE)
Appl. No. 17/625,395
Filed by SYMRISE AG, Holzminden (DE)
PCT Filed Jul. 8, 2019, PCT No. PCT/EP2019/068224 § 371(c)(1), (2) Date Jan. 7, 2022, PCT Pub. No. WO2021/004614, PCT Pub. Date Jan. 14, 2021.
Prior Publication US 2023/0212616 A1, Jul. 6, 2023
Int. Cl. C12N 9/04 (2006.01); C12P 7/02 (2006.01); C12P 7/26 (2006.01)

CPC C12P 7/02 (2013.01) [C12N 9/0006 (2013.01); C12P 7/26 (2013.01); C12Y 101/0105 (2013.01)]

6 Claims

1. A method for the production of a trans-diol or a cis-diol or a hydroxyketone comprising the step(s)

(i) conversion of a trans-diol or a cis-diol to a hydroxyketone catalyzed by an enzyme, which is encoded by a nucleic acid sequence of SEQ ID NO: 1 or a nucleic acid sequence having at least 90% sequence identity to SEQ ID NO: 1, or wherein the enzyme comprises the amino acid sequence of SEQ ID NO: 2, or an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 2, and/or

(ii) conversion of a hydroxyketone to a trans diol or a cis-diol catalyzed by an enzyme as defined in step (i); and

wherein the two hydroxyl groups of the trans-diol and/or the cis-diol is/are attached to an aliphatic ring system comprising 2 to 4 bridged or non-bridged rings;

wherein the trans-diol is (8R,9R)-trans-cedrene diol, the cis-diol is (8R,9S)-cis-cedrene diol and the hydroxyketone is cedrene hydroxyketone.