US 12,281,340 B2
Method of producing N-acyl-amino group-containing compound
Hidemi Nagata, Kanagawa (JP); Keiko Danjou, Kanagawa (JP); Jun Takakura, Kanagawa (JP); and Hiroyuki Nozaki, Kanagawa (JP)
Assigned to AJINOMOTO CO., INC., Tokyo (JP)
Filed by AJINOMOTO CO., INC., Tokyo (JP)
Filed on Sep. 18, 2020, as Appl. No. 17/025,173.
Application 17/025,173 is a continuation of application No. PCT/JP2019/007681, filed on Feb. 27, 2019.
Claims priority of application No. 2018-077741 (JP), filed on Apr. 13, 2018.
Prior Publication US 2021/0071214 A1, Mar. 11, 2021
Int. Cl. C12P 13/00 (2006.01); C12N 9/00 (2006.01); C12N 9/42 (2006.01); C12N 9/80 (2006.01); C12P 21/00 (2006.01)
CPC C12P 13/005 (2013.01) [C12N 9/2445 (2013.01); C12N 9/80 (2013.01); C12N 9/93 (2013.01); C12P 21/00 (2013.01); C12Y 302/01021 (2013.01); C12Y 305/01014 (2013.01); C12Y 602/0103 (2013.01)] 14 Claims
 
1. A method of producing a compound containing an N-acyl-amino group, the method comprising:
reacting a compound containing an amino group with a fatty acid in a culture medium comprising a microorganism, wherein the microorganism produces an enzyme, and said reacting results in production of the compound containing an N-acyl-amino group, wherein the fatty acid is a saturated fatty acid having 6 to 18 carbon atoms, and
isolating the compound containing an N-acyl-amino group from the culture medium,
wherein the enzyme has an ability to bond a carboxyl group and an amino group in an ATP dependent manner to form an amide bond, wherein the enzyme is selected from the group consisting of:
(A) a protein comprising an amino acid sequence selected from the group consisting of SEQ ID NOs: 1 to 9,
(B) a protein comprising an amino acid sequence containing one to twenty amino acid substitutions, deletions, insertions, or additions in the amino acid sequence selected from the group consisting of SEQ ID NOs: 1 to 9, and said protein having an N-acylase activity, and
(C) a protein comprising an amino acid sequence having 95% or more identity to the amino acid sequence selected from the group consisting of SEQ ID NOs: 1 to 9, and said protein having an N-acylase activity.