	US 12,281,333 B2
	Renal tube assay device and methods of manufacture and use
Andras Czirok, Roeland Park, KS (US); and Pamela Tran, Overland Park, KS (US)
Assigned to UNIVERSITY OF KANSAS, Lawrence, KS (US)
Filed by The University of Kansas, Lawrence, KS (US)
Filed on Nov. 3, 2021, as Appl. No. 17/518,395.
Claims priority of provisional application 63/109,630, filed on Nov. 4, 2020.
Prior Publication US 2022/0135951 A1, May 5, 2022
Int. Cl. C12N 5/071 (2010.01); C12M 1/12 (2006.01); C12M 1/34 (2006.01); C12M 3/06 (2006.01); G01N 33/50 (2006.01)

CPC C12N 5/0686 (2013.01) [C12M 23/06 (2013.01); C12M 23/16 (2013.01); C12M 41/36 (2013.01); C12M 41/46 (2013.01); G01N 33/5044 (2013.01)]

20 Claims

1. A renal tube assay device comprising:

a container having an inlet port and an outlet port;

a matrix material in the container, therein the container is located in a cell culture dish; and

a lumen in the matrix material extending from the inlet port to the outlet port,

wherein the lumen includes a luminal surface with at least one low density region that has a lower density compared to another adjacent region of the matrix material that is located at least partially around the at least one low density region.