	US 12,281,320 B2
	Methods and compositions for antibody-evading virus vectors
Aravind Asokan, Chapel Hill, NC (US); Patrick Havlik, Carrboro, NC (US); Mavis Agbandje-McKenna, Gainesville, FL (US); James K. Smith, Gainesville, FL (US); and Mario Mietzsch, Gainesville, FL (US)
Assigned to The University of North Carolina at Chapel Hill, Chapel Hill, NC (US); and University of Florida Research Foundation, Incorporated, Gainsville, FL (US)
Appl. No. 16/976,408
Filed by The University of North Carolina at Chapel Hill, Chapel Hill, NC (US); and University of Florida Research Foundation, Incorporated, Gainsville, NC (US)
PCT Filed Feb. 28, 2019, PCT No. PCT/US2019/020053 § 371(c)(1), (2) Date Aug. 27, 2020, PCT Pub. No. WO2019/169132, PCT Pub. Date Sep. 6, 2019.
Claims priority of provisional application 62/687,583, filed on Jun. 20, 2018.
Claims priority of provisional application 62/636,598, filed on Feb. 28, 2018.
Prior Publication US 2023/0242938 A1, Aug. 3, 2023
Int. Cl. C12N 15/86 (2006.01)

CPC C12N 15/86 (2013.01) [C12N 2750/14122 (2013.01); C12N 2750/14142 (2013.01)]

18 Claims

1. An adeno-associated virus (AAV) capsid protein, wherein the capsid protein comprises a substitution, wherein the substitution comprises the amino acid sequence of RSTFNGDKLN (hi-C: SEQ ID NO:193) at the amino acids corresponding to amino acid positions 661 to 670 of the native AAV8 capsid protein (VP1 numbering) based on the reference amino acid sequence of AAV8 identified as SEQ ID NO:8.