US 12,281,319 B2
Molecular tools and methods for transgene integration and their transposition dependent expression
Jean Livet, Paris (FR); Takuma Kumamoto, Courbevoie (FR); Raphaëlle Barry-Martinet, La Crau (FR); Samuel Tozer, Paris (FR); Franck Maurinot, Issy-les-Moulineaux (FR); Karine Loulier-Le Franc, Paris (FR); Mickaël Le, Maisons Alfort (FR); Stéphane Nedelec, Montrouge (FR); and Michel Cohen-Tannoudji, Montrouge (FR)
Assigned to SORBONNE UNIVERSITE, Paris (FR); INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE (INSERM), Paris (FR); and CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, Paris (FR)
Appl. No. 17/055,942
Filed by SORBONNE UNIVERSITE, Paris (FR); INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE (INSERM), Paris (FR); and CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, Paris (FR)
PCT Filed May 17, 2019, PCT No. PCT/EP2019/062877
§ 371(c)(1), (2) Date Nov. 16, 2020,
PCT Pub. No. WO2019/219947, PCT Pub. Date Nov. 21, 2019.
Claims priority of application No. 18305623 (EP), filed on May 18, 2018.
Prior Publication US 2021/0214747 A1, Jul. 15, 2021
Int. Cl. C12N 15/85 (2006.01); C12N 9/12 (2006.01); C12N 15/90 (2006.01)
CPC C12N 15/85 (2013.01) [C12N 9/1241 (2013.01); C12N 15/907 (2013.01); C12N 2800/90 (2013.01); C12N 2830/001 (2013.01); C12Y 207/07 (2013.01)] 8 Claims
 
1. A recombinant vector, comprising:
a promoter;
a gene of interest; and
a set of two sequences consisting of:
a first sequence comprising or consisting of a 5′ ITR of a DNA transposon which is in the sense orientation with respect to the orientation of the promoter, and
a second sequence comprising or consisting of a 3′ ITR of a DNA transposon which is in the antisense orientation with respect to the orientation of the promoter,
wherein one of the first sequence or of the second sequence of said set of two sequences is located between the promoter and at least part of the gene of interest, and wherein said at least part of the sequence of the gene of interest is in an anti-sense orientation with respect to the orientation of the promoter.