US 12,281,178 B2
Tuning of pore-forming peptides for increasing pore size, membrane affinity, stability, and antimicrobial activity
Aziz Fennouri, Fribourg (CH); Jonathan List, Fribourg (CH); and Michael Mayer, Fribourg (CH)
Assigned to ADOLPHE MERKLE INSTITUTE, UNIVERSITY OF FRIBOURG, Fribourg (CH)
Appl. No. 17/278,765
Filed by ADOLPHE MERKLE INSTITUTE, UNIVERSITY OF FRIBOURG, Fribourg (CH)
PCT Filed Oct. 4, 2019, PCT No. PCT/EP2019/076974
§ 371(c)(1), (2) Date Mar. 23, 2021,
PCT Pub. No. WO2020/074399, PCT Pub. Date Apr. 16, 2020.
Claims priority of provisional application 62/742,579, filed on Oct. 8, 2018.
Prior Publication US 2022/0033527 A1, Feb. 3, 2022
Int. Cl. G01N 33/487 (2006.01); C07K 14/435 (2006.01); C07K 19/00 (2006.01)
CPC C07K 19/00 (2013.01) [C07K 14/43577 (2013.01)] 21 Claims
OG exemplary drawing
 
1. A hybrid pore-forming compound, comprising:
a pore-forming peptide having a first terminus and a second terminus, wherein a first oligonucleotide is linked to the first terminus, wherein the first oligonucleotide is derived from DNA, RNA, LNA, BNA, or PNA, wherein a first functional moiety is linked to the second terminus, and wherein the first functional moiety is hydrophilic,
wherein the pore-forming peptide comprises a Ceratotoxin A (CtxA),
wherein the first oligonucleotide comprises a single strand oligonucleotide, and
wherein the first functional moiety comprises a polythymine strand.