US 12,281,137 B2
Method for the site-specific enzymatic labelling of nucleic acids in vitro by incorporation of unnatural nucleotides
Floyd E. Romesberg, La Jolla, CA (US); Denis A. Malyshev, Solana Beach, CA (US); Lingjun Li, San Diego, CA (US); Thomas Lavergne, Le Versoud (FR); and Zhengtao Li, Pudong new district (CN)
Assigned to The Scripps Research Institute, La Jolla, CA (US)
Filed by The Scripps Research Institute, La Jolla, CA (US)
Filed on Feb. 22, 2023, as Appl. No. 18/112,952.
Application 18/112,952 is a division of application No. 16/518,715, filed on Jul. 22, 2019, granted, now 11,634,451.
Application 16/518,715 is a division of application No. 14/910,203, granted, now 10,626,138, previously published as PCT/US2014/050423, filed on Aug. 8, 2014.
Claims priority of provisional application 61/863,649, filed on Aug. 8, 2013.
Prior Publication US 2024/0101590 A1, Mar. 28, 2024
Int. Cl. C07H 19/24 (2006.01); C07H 19/00 (2006.01); C07H 21/00 (2006.01); C07H 21/04 (2006.01); C12N 9/22 (2006.01); C12N 15/10 (2006.01); C12P 19/34 (2006.01); C12Q 1/6832 (2018.01)
CPC C07H 19/24 (2013.01) [C07H 19/00 (2013.01); C07H 21/00 (2013.01); C07H 21/04 (2013.01); C12N 9/22 (2013.01); C12N 15/1024 (2013.01); C12P 19/34 (2013.01); C12Q 1/6832 (2013.01)] 35 Claims
OG exemplary drawing
 
1. A nucleobase pair comprising a first nucleobase analog having the structure β9b, and a second nucleobase analog having any of the structures α15a or α15b:

OG Complex Work Unit Chemistry
wherein each X is independently carbon or nitrogen,
wherein each R1 is independently hydrogen, alkyl group, a reactive linker comprising a reactive center a linker coupled to a cargo,
wherein each R2 is optional and when present is independently hydrogen, alkyl, alkenyl, alkynyl, methoxy, methanethiol, methaneseleno, halogen, cyano, azide, nitro group, a reactive linker comprising a reactive center, or a linker coupled to a cargo,
wherein each Y is independently sulfur, oxygen, selenium, or a secondary amine,
wherein each E is independently oxygen, sulfur or selenium,
and the wavy line indicates a point of bonding to a ribosyl, deoxyribosyl, or dideoxyribosyl moiety or an analog thereof, wherein the ribosyl, deoxyribosyl, or dideoxyribosyl moiety, or analog thereof is in free form, is bound to a mono-phosphate, diphosphate, triphosphate, α-thiotriphosphate, β-thiophosphate, or γ-thiophosphate group, or is included in an RNA or a DNA or in an RNA analog or a DNA analog.