US 12,280,374 B2
Nanofluidic analytical devices and methods of using thereof
Steven A. Soper, Baton Rouge, LA (US); Collin J. McKinney, Durham, NC (US); Elizabeth Podlaha-Murphy, Potsdam, NY (US); and Sunggook Park, Baton Rouge, LA (US)
Assigned to University of Kansas, Lawrence, KS (US); Univ. of N Carolina at Chapel Hill, Chapel Hill, NC (US); Board of Supervisors of LSU and A&M College, Baton Rouge, LA (US); Northeastern University, Boston, MA (US); and Clarkson University, Potsdam, NY (US)
Appl. No. 17/260,125
Filed by UNIVERSITY OF KANSAS, Lawrence, KS (US); THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL, Chapel Hill, NC (US); BOARD OF SUPERVISORS OF LOUISIANA STATE UNIVERSITY AND AGRICULTURAL AND MECHANICAL COLLEGE, Baton Rouge, LA (US); NORTHEASTERN UNIVERSITY, Boston, MA (US); and CLARKSON UNIVERSITY, Potsdam, NY (US)
PCT Filed Jul. 15, 2019, PCT No. PCT/US2019/041872
§ 371(c)(1), (2) Date Jan. 13, 2021,
PCT Pub. No. WO2020/014708, PCT Pub. Date Jan. 16, 2020.
Claims priority of provisional application 62/714,507, filed on Aug. 3, 2018.
Claims priority of provisional application 62/697,916, filed on Jul. 13, 2018.
Prior Publication US 2021/0268503 A1, Sep. 2, 2021
Int. Cl. B01L 3/00 (2006.01); C12Q 1/6869 (2018.01); G01N 33/68 (2006.01)
CPC B01L 3/502761 (2013.01) [B01L 3/502715 (2013.01); C12Q 1/6869 (2013.01); G01N 33/6818 (2013.01); B01L 2200/0652 (2013.01); B01L 2300/0645 (2013.01); B01L 2300/0896 (2013.01); B01L 2400/0415 (2013.01); B01L 2400/0421 (2013.01)] 18 Claims
OG exemplary drawing
 
1. An analytical device comprising:
(a) a sample processing region comprising two or more fluidically coupled sample handling elements defined by a substrate, wherein the two or more sample handling elements together affect a physical change on a sample introduced into the sample processing region; and
(b) a first nanochannel formed in the substrate and fluidically coupled to the sample processing region, the first nanochannel comprising an input end of the first nanochannel, an output end of the first nanochannel, a first nanopore proximate to the input end of the first nanochannel, and a second nanopore spaced apart from the first nanopore and proximate to the output end of the first nanochannel, and
(c) a second nanochannel coupled orthogonally with the first nanochannel, wherein the second nanochannel comprises an input end of the second nanochannel, an output end of the second nanochannel, a third nanopore proximate to the input end of the second nanochannel, and a fourth nanopore spaced apart from the third nanopore and proximate to the output end of the second nanochannel.