US 12,280,370 B2
Covalently modified surfaces, kits, and methods of preparation and use
Randall D. Lowe, Jr., Emeryville, CA (US); Alexander J. Mastroianni, Alameda, CA (US); Mark P. White, Orinda, CA (US); Gregory G. Lavieu, Vitry sur Seine (FR); and Kristin G. Beaumont, New York, NY (US)
Assigned to Bruker Cellular Analysis, Inc., Emeryville, CA (US)
Filed by BRUKER CELLULAR ANALYSIS, INC., Emeryville, CA (US)
Filed on Sep. 13, 2023, as Appl. No. 18/466,696.
Application 18/466,696 is a continuation of application No. 17/162,467, filed on Jan. 29, 2021, granted, now 11,801,508.
Application 17/162,467 is a continuation of application No. 16/196,649, filed on Nov. 20, 2018, granted, now 11,007,520, issued on May 18, 2021.
Application 16/196,649 is a continuation of application No. PCT/US2017/034832, filed on May 26, 2017.
Claims priority of provisional application 62/411,191, filed on Oct. 21, 2016.
Claims priority of provisional application 62/410,238, filed on Oct. 19, 2016.
Claims priority of provisional application 62/353,938, filed on Jun. 23, 2016.
Claims priority of provisional application 62/345,603, filed on Jun. 3, 2016.
Claims priority of provisional application 62/342,131, filed on May 26, 2016.
Prior Publication US 2024/0375097 A1, Nov. 14, 2024
Int. Cl. B01L 3/00 (2006.01); B81C 1/00 (2006.01); C12M 3/06 (2006.01); G01N 33/543 (2006.01)
CPC B01L 3/502707 (2013.01) [B01L 3/502715 (2013.01); B01L 3/502761 (2013.01); B81C 1/00206 (2013.01); C12M 23/16 (2013.01); G01N 33/54393 (2013.01); B01L 2300/0636 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0864 (2013.01); B01L 2300/088 (2013.01); B81B 2201/058 (2013.01)] 13 Claims
OG exemplary drawing
 
1. A kit including:
a microfluidic device comprising: an enclosure including a base, a cover, and microfluidic circuit material defining a fluidic circuit therein, where at least one inner surface of the base, the cover and the microfluidic circuit material has a plurality of first covalently bound surface modifications, each including a first linking group and a first moiety, where the first moiety is a first reactive moiety or a first surface contact moiety;
where at least one inner surface of the base, the cover and the microfluidic circuit material has a plurality of second covalently bound surface modifications, each including a second linking group and a second moiety, where the second moiety is a second reactive moiety or a second surface contact moiety; and
a first surface modifying reagent having a structure of Formula XII1:
RP1-L1-surface contact moiety1  (Formula XII1)
wherein RP1 is a reaction pair moiety; surface contact moiety1 is a moiety configured to support cell growth, viability, portability, or any combination thereof; and L1 is a linker; wherein L1 is a bond or a chain of 1 to 200 non-hydrogen atoms selected from any combination of silicon, carbon, nitrogen, oxygen, sulfur and phosphorus atoms, and may further include 0 or 1 coupling groups CG.