US 12,280,176 B2
Methods and compositions for matrix preparation
Sherry L. Voytik-Harbin, Zionsville, IN (US)
Assigned to GENIPHYS, INC., Zionsville, IN (US)
Appl. No. 16/482,465
Filed by GENIPHYS, INC., Zionsville, IN (US)
PCT Filed Jan. 31, 2018, PCT No. PCT/US2018/016069
§ 371(c)(1), (2) Date Jul. 31, 2019,
PCT Pub. No. WO2018/144496, PCT Pub. Date Aug. 9, 2018.
Claims priority of provisional application 62/452,564, filed on Jan. 31, 2017.
Prior Publication US 2020/0246507 A1, Aug. 6, 2020
Int. Cl. A61L 27/36 (2006.01); A61L 2/00 (2006.01); C07K 14/78 (2006.01)
CPC A61L 27/3633 (2013.01) [A61L 2/0047 (2013.01); A61L 27/3683 (2013.01); C07K 14/78 (2013.01)] 19 Claims
 
1. A method for preparing a matrix, said method comprising polymerizing oligomeric collagen, using a single mixing step for the polymerization, said method comprising mixing an oligomeric collagen composition with a buffer solution to form a collagen solution wherein the buffer solution comprises a base and less than 00.2 mM MgCl2, wherein the buffer and the base are in the same composition when mixed with the oligomeric collagen composition for polymerization of the oligomeric collagen, and wherein the polymerization of the oligomeric collagen in the collagen solution results in formation of the matrix.