US 12,280,168 B2
Method for sanitization of a chromatography column
Johan Avallin, Uppsala (SE); Anna Gronberg, Uppsala (SE); Anders Nilsson, Uppsala (SE); Henrik Ingvarsson, Uppsala (SE); Linda Persson, Uppsala (SE); Magnus Asplund, Uppsala (SE); and Reinhard Braaz, Penzberg (DE)
Assigned to Cytiva Sweden AB, Uppsala (SE)
Appl. No. 17/437,097
Filed by Cytiva Sweden AB, Uppsala (SE)
PCT Filed Mar. 23, 2020, PCT No. PCT/EP2020/058023
§ 371(c)(1), (2) Date Sep. 8, 2021,
PCT Pub. No. WO2020/193485, PCT Pub. Date Oct. 1, 2020.
Claims priority of application No. 1904125 (GB), filed on Mar. 26, 2019.
Prior Publication US 2022/0175992 A1, Jun. 9, 2022
Int. Cl. A61L 2/18 (2006.01); B01D 15/20 (2006.01); B01D 15/38 (2006.01); G01N 30/50 (2006.01); G01N 30/56 (2006.01); G01N 30/60 (2006.01); A61L 101/36 (2006.01)
CPC A61L 2/186 (2013.01) [B01D 15/203 (2013.01); B01D 15/3809 (2013.01); G01N 30/50 (2013.01); G01N 30/56 (2013.01); G01N 30/6021 (2013.01); G01N 30/6026 (2013.01); A61L 2101/36 (2020.08); A61L 2202/23 (2013.01)] 18 Claims
OG exemplary drawing
 
1. A method for sanitization of a bioprocess chromatography column, comprising the steps of:
a) providing a bioprocess chromatography column with a packed bed of separation resin particles and a movable adapter;
b) with the adapter in contact with the packed bed, conveying a sanitization fluid through the packed bed to a column outlet;
b′) closing and opening a column resin valve;
c) raising the adapter to provide a gap between the packed bed and the adapter;
d) lowering the adapter to close the gap;
d′) conveying the sanitization fluid through the packed bed to the column outlet; and
e) conveying an equilibration liquid through the packed bed to a column outlet.