US 12,280,136 B2
Compositions containing exosomes from animal placenta, methods for producing the same and uses thereof
Haijia Chen, Guangdong (CN); Jiaohua Jiang, Guangdong (CN); Xuejia Li, Guangdong (CN); Jiwang Xu, Guangdong (CN); Keliang Pang, Guangdong (CN); Donghuang Chen, Guangdong (CN); Aimei Zhou, Guangdong (CN); Li Tao, Guangdong (CN); Zihao Cui, Guangdong (CN); Baoyao Li, Guangdong (CN); and Xinming Yan, Guangdong (CN)
Assigned to GUANGZHOU SALIAI BIOLOGICAL GENETIC ENGINEERING CO., LTD., Guangdong (CN); and GUANGZHOU SALIAI STEM CELL SCIENCE AND TECHNOLOGY CO., LTD., Guangdong (CN)
Filed by GUANGZHOU SALIAI BIOLOGICAL GENETIC ENGINEERING CO., LTD., Guangdong (CN); and GUANGZHOU SALIAI STEM CELL SCIENCE AND TECHNOLOGY CO., LTD., Guangdong (CN)
Filed on Mar. 15, 2022, as Appl. No. 17/694,715.
Claims priority of application No. 202110275136.3 (CN), filed on Mar. 15, 2021; application No. 202110275137.8 (CN), filed on Mar. 15, 2021; and application No. 202110325589.2 (CN), filed on Mar. 26, 2021.
Prior Publication US 2022/0287952 A1, Sep. 15, 2022
Int. Cl. A61K 8/92 (2006.01); A61K 8/55 (2006.01); A61K 8/67 (2006.01); A61K 8/73 (2006.01); A61K 8/86 (2006.01); A61Q 19/08 (2006.01)
CPC A61K 8/925 (2013.01) [A61K 8/553 (2013.01); A61K 8/678 (2013.01); A61K 8/738 (2013.01); A61K 8/86 (2013.01); A61Q 19/08 (2013.01); A61K 2800/622 (2013.01); A61K 2800/624 (2013.01); A61K 2800/65 (2013.01); A61K 2800/84 (2013.01)] 1 Claim
 
1. A method for preparing exosomes from bovine placenta comprising:
homogenizing bovine placenta to obtain a homogenate, and subjecting the homogenate to freeze-thaw process, wherein the freeze-thaw process comprises freezing at −80° C. for 24 h and then thawing at room temperature, and the freeze-thaw process is performed once;
performing a first round of ultrasonic treatment on the thawed homogenate and filtering, and freezing the filtrate at −80° C., wherein power of the first round of ultrasonic treatment is 990 w and time is 30 min;
thawing the filtrate at 4° C. and centrifuging to obtain a supernatant, performing a second round of ultrasonic treatment on the supernatant, and performing multiple filtrations through filters to obtain the exosomes from bovine placenta;
wherein the second round of ultrasonic treatment is performed once at an interval of 8 s, each ultrasonic treatment lasts for 8 s, the power of the second round of ultrasonic treatment is 500 w, and the total time of ultrasonic treatment is 30 min; and
the multiple filtrations are four filtrations, through filter meshes with pore sizes of 5 μm, 1 μm, 0.45 μm and 0.22 μm in sequence.