US 12,280,123 B2
Recombinant adenoviruses carrying transgenes
Christopher Larson, San Diego, CA (US); Bryan Oronsky, Los Altos Hills, CA (US); and Tony R. Reid, San Diego, CA (US)
Assigned to EPICENTRX, INC., La Jolla, CA (US)
Filed by EpicentRx, Inc., La Jolla, CA (US)
Filed on Jan. 13, 2022, as Appl. No. 17/575,488.
Application 17/575,488 is a continuation of application No. 15/991,745, filed on May 29, 2018, granted, now 11,253,608.
Claims priority of provisional application 62/511,822, filed on May 26, 2017.
Prior Publication US 2022/0125946 A1, Apr. 28, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 35/761 (2015.01); A61K 47/68 (2017.01); A61K 47/69 (2017.01); A61K 48/00 (2006.01); A61P 35/00 (2006.01); C12N 7/02 (2006.01); C12N 7/04 (2006.01); C12N 15/62 (2006.01); C12N 15/85 (2006.01); C12N 15/86 (2006.01)
CPC A61K 47/6829 (2017.08) [A61K 35/761 (2013.01); A61K 47/6851 (2017.08); A61K 47/6901 (2017.08); A61K 48/0025 (2013.01); A61K 48/0066 (2013.01); A61K 48/0083 (2013.01); A61P 35/00 (2018.01); C12N 7/025 (2013.01); C12N 7/045 (2013.01); C12N 15/62 (2013.01); C12N 15/85 (2013.01); C12N 15/86 (2013.01); C12N 2710/10343 (2013.01)] 17 Claims
 
1. A recombinant adenovirus comprising a nucleotide sequence inserted in an IX-E2 insertion site, wherein the IX-E2 insertion site is located between the stop codon of adenovirus IX gene and the stop codon of adenovirus IVa2 gene, wherein the nucleotide sequence comprises a promoter, a transgene, and a second polyadenylation signal and the recombinant adenovirus comprises in a 5′ to 3′ orientation:
(i) a first polyadenylation signal;
(ii) a fourth polyadenylation signal;
(iii) the promoter;
(iv) the transgene;
(v) the second polyadenylation signal; and
(vi) a third polyadenylation signal;
wherein the transgene is operably linked to the promoter,
wherein the second polyadenylation signal is the polyadenylation signal of the transgene, and
wherein the fourth polyadenylation signal is in the opposite transcriptional direction of the first polyadenylation signal.