US 12,280,114 B2
Extraction of plant source “medicinal soup” and manual preparation of “herbal medicine” and related products
Chengyu Jiang, Beijing (CN); Xiaoyun Li, Beijing (CN); Jianchao Du, Beijing (CN); Zhu Liang, Beijing (CN); Zhiqing Wang, Beijing (CN); and Chenxuan Wang, Beijing (CN)
Assigned to INSTITUTE OF BASIC MEDICAL SCIENCES, Beijing (CN)
Appl. No. 17/042,924
Filed by BEIJING BAISHIHEKANG PHARMACEUTICAL TECHNOLOGY (BSJPHARMA) CO., LTD, Beijing (CN)
PCT Filed Mar. 5, 2019, PCT No. PCT/CN2019/077004
§ 371(c)(1), (2) Date Sep. 29, 2020,
PCT Pub. No. WO2019/184663, PCT Pub. Date Oct. 3, 2019.
Claims priority of application No. PCT/CN2018/081155 (WO), filed on Mar. 29, 2018.
Prior Publication US 2021/0113641 A1, Apr. 22, 2021
Int. Cl. A61K 47/14 (2017.01); A61K 36/288 (2006.01); A61K 36/41 (2006.01); A61K 45/06 (2006.01); B82Y 5/00 (2011.01); B82Y 40/00 (2011.01); C12N 15/113 (2010.01)
CPC A61K 47/14 (2013.01) [A61K 36/288 (2013.01); A61K 36/41 (2013.01); A61K 45/06 (2013.01); C12N 15/113 (2013.01); A61K 2236/331 (2013.01); A61K 2236/333 (2013.01); A61K 2236/37 (2013.01); A61K 2236/51 (2013.01); A61K 2236/53 (2013.01); B82Y 5/00 (2013.01); B82Y 40/00 (2013.01); C12N 2310/14 (2013.01); C12N 2320/32 (2013.01)] 6 Claims
 
1. A method for preparing a bencaosome, comprising the steps of:
(1) mixing Sphinganine (d22:0) with one or more small RNAs;
wherein the Sphinganine (d22:0) is synthesized or purified;
wherein the one or more small RNAs have a length of 14-32 bp;
(2) treating the mixture obtained from step (1) by heating,
wherein the heating temperature is from about 80° C. to about 100° C.;
wherein the time for heating is about 15 minutes to about 30 minutes;
wherein the mixing is performed by adding a solution of the Sphinganine (d22:0) in an organic solvent into an aqueous solution of the small RNA;
wherein the bencaosome is a nano-particulate substance with a membrane structure.