US 12,280,112 B2
Magnetic delivery system and hydrogel fixative for therapeutic cells
David A. Vorp, Pittsburgh, PA (US); Aneesh Krishna Ramaswamy, Pittsburgh, PA (US); Justin Sol Weinbaum, Allison Park, PA (US); Kory James Blose, Pittsburgh, PA (US); Timothy Kwang-Joon Chung, Pittsburgh, PA (US); Trevor Kickliter, Allentown, PA (US); Yogev Baruch, Pittsburgh, PA (US); and John A. Curci, Nashville, TN (US)
Assigned to University of Pittsburgh—Of the Commonwealth System of Higher Education, Pittsburgh, PA (US); and Vanderbilt University, Nashville, TN (US)
Appl. No. 17/286,210
Filed by University of Pittsburgh—Of the Commonwealth System of Higher Education, Pittsburgh, PA (US); and Vanderbilt University, Nashville, TN (US)
PCT Filed Oct. 18, 2019, PCT No. PCT/US2019/056976
§ 371(c)(1), (2) Date Apr. 16, 2021,
PCT Pub. No. WO2020/081957, PCT Pub. Date Apr. 23, 2020.
Claims priority of provisional application 62/747,767, filed on Oct. 19, 2018.
Prior Publication US 2021/0338816 A1, Nov. 4, 2021
Int. Cl. A61K 41/00 (2020.01); A61K 35/28 (2015.01); A61M 5/14 (2006.01); A61M 25/01 (2006.01); A61M 25/09 (2006.01); C12N 5/0775 (2010.01)
CPC A61K 41/00 (2013.01) [A61K 35/28 (2013.01); A61M 5/1407 (2013.01); A61M 25/0127 (2013.01); A61M 25/09041 (2013.01); C12N 5/0667 (2013.01); A61M 2202/09 (2013.01); A61M 2205/0288 (2013.01)] 15 Claims
OG exemplary drawing
 
1. A method of delivering a cell to an aortic aneurysm, comprising:
mixing cells comprising internalized magnetic particles with a hydrogel pre-gel composition;
depositing the pre-gel composition comprising the cells at a site on or adjacent to a tissue, under conditions suitable for gelation of the pre-gel composition such that the pre-gel composition forms a hydrogel at the site; and
prior or during gelation of the hydrogel, applying a magnetic field to the cells in the deposited pre-gel to draw the cells to the aneurysm.