	US 12,280,099 B2
	Dengue virus vaccine
Michinori Kohara, Tokyo (JP); Fumihiko Yasui, Tokyo (JP); Daisuke Yamane, Tokyo (JP); Kyoko Kohara, Kagoshima (JP); Kouichi Morita, Nagasaki (JP); Yasuhiro Yasutomi, Ibaraki (JP); and Koji Ishii, Tokyo (JP)
Assigned to TOKYO METROPOLITAN INSTITUTE OF MEDICAL SCIENCE, Tokyo (JP); KAGOSHIMA UNIVERSITY, Kagoshima (JP); and NAGASAKI UNIVERSITY, Nagasaki (JP)
Appl. No. 17/438,178
Filed by TOKYO METROPOLITAN INSTITUTE OF MEDICAL SCIENCE, Tokyo (JP); KAGOSHIMA UNIVERSITY, Kagoshima (JP); and NAGASAKI UNIVERSITY, Nagasaki (JP)
PCT Filed Mar. 13, 2020, PCT No. PCT/JP2020/012569 § 371(c)(1), (2) Date Oct. 1, 2021, PCT Pub. No. WO2020/184730, PCT Pub. Date Sep. 17, 2020.
Claims priority of application No. 2019-047582 (JP), filed on Mar. 14, 2019.
Prior Publication US 2022/0152187 A1, May 19, 2022
Int. Cl. A61K 39/12 (2006.01); A61P 31/14 (2006.01); C12N 15/113 (2010.01); A61K 39/00 (2006.01)

CPC A61K 39/12 (2013.01) [A61P 31/14 (2018.01); C12N 15/1131 (2013.01); A61K 2039/5254 (2013.01)]

11 Claims

1. A recombinant vaccinia virus comprising a cDNA encoding a non-structural protein from a dengue virus, and an expression promoter,

wherein the cDNA encoding a non-structural protein is DNA of (a)-(e) below:

(a) DNA coding a region consisting of NS2A, NS2B, NS3, NS4A, NS4B and NS5 regions;

(b) DNA consisting of the nucleotide sequence represented by SEQ ID NO:2;

(c) DNA which has 99% or higher identity with DNA consisting of a nucleotide sequence complementary to the nucleotide sequence represented by SEQ ID NO:2, and which codes for a non-structural protein from a dengue virus;

(d) DNA consisting of the nucleotide sequence represented by SEQ ID NO:3; and

(e) DNA which has 95% or higher identity with DNA consisting of a nucleotide sequence complementary to the nucleotide sequence represented by SEQ ID NO:3, and which codes for a non-structural protein from a dengue virus.