US 12,280,074 B2
Preparation of platelet pellet lysate and its use for treating neurological disorders
David Devos, Lille (FR); Thierry Burnouf, Lille (FR); Jean-christophe Devedjian, Lille (FR); and Ming-Li Chou, Taoyuan (TW)
Assigned to Centre Hospitalier Regional et Universitaire de Lille (CHRU), Lille (FR); Universite de Lille, Lille (FR); Universite du Littoral Cote D'Opale, Dunkirk (FR); Inserm (Institut National de La Sante et de la Recherche Medicale), Paris (FR); and Taipei Medical University, Taipei (TW)
Appl. No. 16/087,471
Filed by CENTRE HOSPITALIER REGIONAL ET UNIVERSITAIRE DE LILLE (CHRU), Lille (FR); UNIVERSITE DE LILLE, Lille (FR); UNIVERSITE DU LITTORAL COTE D'OPALE, Dunkirk (FR); INSERM (INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE), Paris (FR); and TAIPEI MEDICAL UNIVERSITY, Taipei (TW)
PCT Filed Mar. 23, 2017, PCT No. PCT/EP2017/057004
§ 371(c)(1), (2) Date Sep. 21, 2018,
PCT Pub. No. WO2017/162830, PCT Pub. Date Sep. 28, 2017.
Claims priority of application No. 16305332 (EP), filed on Mar. 23, 2016.
Prior Publication US 2019/0099448 A1, Apr. 4, 2019
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 35/19 (2015.01); A61P 25/00 (2006.01); A61P 25/16 (2006.01); A61P 25/28 (2006.01)
CPC A61K 35/19 (2013.01) [A61P 25/16 (2018.01); A61P 25/28 (2018.01)] 25 Claims
 
1. A method for treating a neurological disorder selected from Parkinson's disease and amyotrophic lateral sclerosis, comprising a step of administering an effective amount of a platelet pellet lysate which is modified by a heat treatment at a temperature of 55° C. to 65° C. for 20 to 40 minutes to a patient in need thereof, said lysate having a total protein content of less than 70% of the total protein content of non-heated platelet pellet lysate, wherein the platelet pellet lysate is prepared using a method comprising steps of:
i. providing a platelet concentrate from human blood;
ii. centrifuging the platelet concentrate to obtain a platelet pellet and a plasma supernatant;
iii. removing the plasma supernatant and suspending the platelet pellet;
iv. subjecting the suspended platelet pellet to freeze/thaw cycles to lyse platelets of the suspended platelet pellet and obtain a suspension; and
v. removing cell debris of the suspension obtained in step iv by centrifugation to obtain a platelet pellet lysate.