	US 12,279,600 B2
	Genetically modified sterile avians and method for the reconstitution thereof
Mike McGrew, Edinburgh Lothian (GB); and Mark Woodcock, Edinburgh Lothian (GB)
Assigned to The University Court of the University of Edinburgh, Edinburgh (GB)
Appl. No. 17/282,846
Filed by The University Court of the University of Edinburgh, Edinburgh Lothian (GB)
PCT Filed Oct. 11, 2019, PCT No. PCT/GB2019/052895 § 371(c)(1), (2) Date Apr. 5, 2021, PCT Pub. No. WO2020/074915, PCT Pub. Date Apr. 16, 2020.
Claims priority of application No. 1816633 (GB), filed on Oct. 12, 2018.
Prior Publication US 2021/0345592 A1, Nov. 11, 2021
Int. Cl. A01K 67/0275 (2024.01); C12N 15/85 (2006.01)

CPC A01K 67/0275 (2013.01) [C12N 15/8509 (2013.01); A01K 2217/15 (2013.01); A01K 2217/20 (2013.01); A01K 2217/30 (2013.01); A01K 2227/30 (2013.01); A01K 2267/02 (2013.01)]

11 Claims

1. A transgene construct comprising:

(i) a first nucleotide sequence, wherein said first nucleotide sequence encodes an apoptosis inducing enzyme, wherein said apoptosis inducing enzyme is inducible Caspase9 (iCaspase9), wherein the activity of the protein encoded by said first nucleotide sequence causes death of germ cells in the presence of an exogenous induction agent; and

(ii) a second nucleotide sequence which targets said construct to avian germ cells, wherein said second nucleotide sequence targets the genetic DAZL locus.